摘要
为明确2株生防细菌的分类地位,采用传统形态学方法结合Biolog微生物自动分析系统,鉴定了大豆根腐病的2株生防细菌。结果表明,菌株B021a与塔式弧菌相似度值为0.634,可能性是86%,遗传距离为4.00。菌株B04b与海藻巴斯德菌相似度值为0.610,可能性是75%,遗传距离为2.77。综合形态学和Biolog鉴定结果,认为菌株B021a是塔式弧菌,菌株B04b是海藻巴斯德菌。
In order to identify the systematic position of taxonomy of two biocontrol bacteria against soybean root rot. Traditional morphological identification and BIOLOG automatic microbiology analysis system were used to iden- tify strain BO21a and B04b. The results showed that similarity value of strain B021a with Vibrio tubiashii was 0. 634, possibility to 86% and genetic distance to 4.00, and similarity value of strain B04b with PasteureUa trehalosi was 0.610,probability to 75% and genetic distance to 2.77. Strain BO21a was identified as Vibrio tubiashii and strain B04b as Pasteurella trehalosi by colony morphological propertie and BIOLOG analysis system.
出处
《华北农学报》
CSCD
北大核心
2012年第2期234-238,共5页
Acta Agriculturae Boreali-Sinica
基金
国家“十一五”科技支撑计划项目(2006BAD21B01-15)
黑龙江省“十一五”科技攻关项目(GA06B101-1-5)
关键词
大豆
根腐病
拮抗细菌
Biolog自动分析系统
鉴定
Soybean
Root rot disease
Antagonistic bacteria
Biolog automatic microbiology analysis system
Identification
