摘要
目的:以4种中药有效组分配伍的抗氧化活性为研究对象,探索适合中药多组分多指标"组效关系"的研究方法。方法:基于LARS回归算法和综合权重分析方法,按照均匀试验设计-药效试验-数学建模(模型验证)-综合药效评价程序,进行甘草总黄酮、银杏叶提取物、淫羊藿总黄酮和黄芪总苷4个中药组分不同配伍剂量体外清除DPPH和多环芳烃的研究。结果:采用改进最小角回归分析和综合权重法,评价清除DPPH和多环芳烃的最优综合药效,中药组分配伍最佳剂量组合为甘草总黄酮-银杏叶提取物-淫羊藿总黄酮-黄芪总苷(1∶0.254 5∶0.007 6∶0.011 5)。结论:上述中药组分配伍能有效清除多环芳烃和DPPH,具有良好的抗氧化作用。本实验采用的均匀设计结合综合权重法,适合"非线性、小样本"生物实验数据分析,是进行中药组分配伍筛选评价的适合研究方法。
Objective： To find a research method suitable to multi-component muhi-index composition-activity relationship of tra- ditional Chinese medicines （TCMs） with the antioxidant activity of four compatible active constituents of TCMs as the subject. Method： LARS-based regression algorithm and comprehensive weight coefficient method were adopted to study in vitro clearance of DPPH and pol- yaromatic hydrocarbon with different doses of four compatible active constituents--total flavonoids of Glycyrrhizae Radix et Rhizoma （TFG）, Ginkgo Folium extract （GBE）, total flavonoids of Epimedii Folium （TFE） and astragaloside （AST） according to the compre- hensive efficacy assessment procedures, that is test design, efficacy test and mathematical modeling （model verification）. Result： LARS and comprehensive weight coefficient method was adopted to assess the optimal comprehensive efficacy of clearing DPPH and polyaromatic hydrocarbon. The resuhs showed that the optimal dose combination of compatible constituents is TFG-GBE-TFE-AST 1 ： O. 254 5 ： 0. 007 6：0.011 5. Conclusion： The above compatibility of TCMs constituents can effectively clear polyaromatic hydrocarbon and DPPH with a good antioxidation. The uniform design adopted in this experiment, in combination of synthetic weight method, is suitable to the data analysis of ＂non-linear and small-sample＂ biotic experiment and the study on screening and assessment of compatibility of TCMs con- stituents.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第12期1826-1829,共4页
China Journal of Chinese Materia Medica
关键词
中药有效组分配伍
均匀设计
LARS回归算法
综合权重法
抗氧化
compatibility of active constituents of traditional Chinese medicines
uniform design
LARS regression
compre-hensive weight coefficient method
antioxidative capacity
