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Ectopic Expression of a Bacterium NhaD-type Na^+/H^+ Antiporter Leads to Increased Tolerance to Combined Salt/Alkali Stresses 被引量:6

Ectopic Expression of a Bacterium NhaD-type Na^+/H^+ Antiporter Leads to Increased Tolerance to Combined Salt/Alkali Stresses
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摘要 AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na+/H+ antiporter crucial for the bacterium's resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaDwere generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na+ concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, AaNhaD.overexpressing tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na+/H+ antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance. AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na+/H+ antiporter crucial for the bacterium's resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaDwere generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na+ concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, AaNhaD.overexpressing tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na+/H+ antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2012年第6期412-421,共10页 植物学报(英文版)
基金 supported by grants from the National Natural Science Foundation(30771162) the Ministry of Agriculture of China(2009ZX08009-096B)
关键词 Alkaliphiles Alkalimonas amylolytica Na+/H+ antiporter tobacco BY-2 cells high salinity stress alkaline. Alkaliphiles Alkalimonas amylolytica Na+/H+ antiporter tobacco BY-2 cells high salinity stress alkaline.
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