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d(TC_n)序列形成i-Motif四聚体的电喷雾质谱分析

Oligonucleotide Forming Tetramolecular i-Motif Structure Studied by Electrospray Ionization Mass Spectrometry
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摘要 富含胞嘧啶(C)的DNA序列d(TCn)能通过C和质子化CH+氢键之间的相互作用形成平行的双链结构,两条质子化双链从头到尾插入,进一步形成反平行i-Motif四链结构。利用电喷雾质谱在乙酸-乙酸铵缓冲溶液中研究了样品浓度、缓冲液的浓度、溶液的pH值及d(TCn)序列中C的长度(n=3,4,5,6)对d(TCn)形成四分子i-Motif结构影响。结果显示:d(TC5)序列在pH 4.0~5.0,甲醇含量为30%,浓度为20mmol/L醋酸-醋酸铵缓冲溶液中形成四聚体准分子离子强度最高。对于所研究的d(TCn)序列,序列长度(n)越大,形成四分子i-Motif离子的相对强度越高。 Cytocine(C) rich DNA sequence oligonucleotide(d(TCn)) can form parallel duplex through the interactions of C and protonated CH+ base pair and two parallel duplexes intercalate into each other in a head to tail orientation to adopt a four-stranded i-Motif structure.In this study,the tetramolecular ions formed by the sequence d(TCn) are measured by electrospray ionization mass spectrometry,and the influence of solution conditions and cytocine length on the formation of tetramolecular i-Motif,are investigated.The results show that d(TC5) sequence in a buffer solution containing 20 mmol/L ammonium acetate and 30% methanol with pH 4.0-5.0 generates the most abundant tetramolecular ion.Increasing n numbers of the d(TCn)(where n varies from 3-6) favors the formation of the tetramolecular i-Motif.
出处 《分析化学》 SCIE CAS CSCD 北大核心 2012年第6期932-935,共4页 Chinese Journal of Analytical Chemistry
基金 国家自然科学基金面上项目(No.20975044) 吉林省国际合作项目(No.20110707)资助
关键词 电喷雾离子阱质谱 四聚体i-Motif结构 d(TCn)序列 C.CH+碱基对 Electrosprary ionization-ion trap mass spectrometry; Tetramolecualr i-Motif structure; Oligonucleotide sequence; C·CH+ base pair
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  • 1Gilbert D E, Feigon J. Current Opinion in Structural Biology. , 1999, 9(3): 305-314.
  • 2Gueron M, Leroy J L. Current Opinion in Structural Biology. , 2000, 10(3):326-331.
  • 3Mills M, Lacroix L, Arimondo P B, Leroy J-L, Francois J-C, Klump H, Mergny J-L. Curr. Ailed. Chem. Anti Cancer Agents. , 2002, 2(5) : 627-644.
  • 4Song G T, Chen M. L, Chen C, Wang C Y, Hu D, Ren J S, Qu X G. Biochemistry, 2010, 92(2):121-127.
  • 5Kaushik M, Prasad M, Kaushik S, Singh A, Kukreti S. Biopolymers, 2010, 93(2):150-160.
  • 6ChenL Q, CaiL, Zhang X H, Rich A. Biochemistry, 1994, 33(46): 13540-13546.
  • 7AlbertiP, MergnyJ L. PNAS, 2003, 100(4): 1569-1573.
  • 8Sarig G, Weisman-Shomer P, Erlitzki R, Fry M. J. Biol. Chem. , 1997, 272:4478-4482.
  • 9Marsich E, Piccini A, Xodo L. E, Manzini G. Nucleic. Acids. Res. , 1996, 24(20):4029-4033.
  • 10Leroy J L. Nucleic Acids Research. , 2009, 37(12):4127-4134.

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