摘要
目的:在小鼠植骨气囊动物模型中,观察外源性FTY720(Fingolimod,芬戈莫德)对骨溶解的抑制作用。方法:构建小鼠植骨气囊模型,将构建好气囊模型的小鼠分成4组:空白组(气囊内注入生理盐水、腹腔注射DMSO),FTY720组(气囊内注入生理盐水、腹腔注射FTY720),聚乙烯颗粒组(气囊内注入聚乙烯颗粒、腹腔注射DMSO),聚乙烯颗粒+FTY720组(气囊内注入聚乙烯颗粒、腹腔注射FTY720)。4周后处死小鼠后取气囊组织进行HE染色观察炎症反应和炎症细胞渗出情况,取组织匀浆行Elisa法检测气囊组织中IL-6和TNF-α浓度、取骨组织行电镜扫描观察骨片吸收情况,取组织匀浆行RT-PCR检测破骨细胞相关基因抗酒石酸酸性磷酸酶(TRAP)mRNA表达水平。结果:①HE染色后炎症细胞渗出量,聚乙烯颗粒+FTY720组(4892±457)与聚乙烯颗粒组(4931±572)比较无统计学差异(P>0.05),FTY720组(2013±375)与空白组(2151±310)比较无统计学差异(P>0.05);含有聚乙烯颗粒组高于无聚乙烯颗粒组(P<0.05)。②Elisa法检测炎症因子(IL-6、TNF-α)浓度,聚乙烯颗粒+FTY720组〔(130.13±3.22)(、129.22±5.02)〕与聚乙烯颗粒组〔(149.22±6.09)(、140.52±2.68)〕比较无统计学差异(P>0.05);FTY720组〔(68.24±2.83)、(82.88±5.34)〕与空白组〔(69.51±2.31)(、74.15±4.64)〕比较没有统计学差异(P>0.05);含有聚乙烯颗粒组高于无聚乙烯颗粒组(P<0.05)。③扫描电镜检测骨片吸收面积与视野面积百分比,聚乙烯颗粒+FTY720组〔(10.21±1.78)%〕低于聚乙烯颗粒组〔(17.79±2.56)%〕,FTY720组〔(1.82±1.37)%〕低于空白组〔(6.27±2.11)%〕;并且含有聚乙烯颗粒组高于无聚乙烯颗粒组(P<0.05)。④破骨细胞相关基因TRAP mRNA表达量,聚乙烯颗粒+FTY720组(0.53±0.04)低于聚乙烯颗粒组(0.74±0.05),FTY720组(0.22±0.03)低于空白组(0.38±0.04);而含有聚乙烯颗粒组高于无聚乙烯颗粒组(P<0.05)。结论:在小鼠植骨气囊动物模型中,FTY720能有效抑制聚乙烯颗粒诱导的局部骨溶解作用。
Objective： To investigate the effect of FTY720（Fingolimod） on osteolysis induced by ultra- high- molecular- weight polyethylene（UHMWPE） particles in mouse air pouch model. Methods： The air pouches model was established, mice were divided into four groups： ultra - high - molecular - weight polyethylene（UttMWPE） particles and FFY720 group （UHMWPE was introduced into the air pouches and mice were injected intraperitoneally with FTFY720）; UHMWPE group（ UHMWPE was introduced into the air pouches and mice were injected intraperituneally with DMSO） ; FTY720 goup （normal saline was introduced into the air pouches and mice were injected intraperitoneally with FFY720） ;blank group（ normal saline was intredueed into the air pouches and mice were injected intraporitoneally with DMSO）. Four weeks later, all the mice are sacrificed, pouch walls and implanted calvaria were collected. and imqanmmtory reaction and the number of exuded inflammatory cells of the pouch walls were detected through HE staining, some of inflanmuitory cytokines, IL - 6 and TNF - a, in tissue homogenate were detected with ELISA; the resorption of the bone slices were detected with scanning electron microscopy; the osteeclast associated gene TRAP in pouch tissues were detected with RT- PCR. Results： （1）HE staining results showed that the number of exuded inflammatfry ceils of the pouch walls in the UHMWPE and FFY720 group （4892 ± 457 ） had no difference compared with the UHMWPE group（ 4931 ± 572 ） （ P 〉 0.05 ）, there was no difference between the FTY720 group （ 2013 ± 375 ） and the blank group （2151 ± 310） （ P 〉 0.05 ） ; but inflammatory cell amounts in the UHMWPE groups were higher than the groups that without treatment UHMWPE （P 〈 0.05 }. （2）ELISA results showed that the quantity of inflannYuatory cytokines IL- 6 and TNF-α tissue hongenate in the UHMWPE and bq＇Y720 group[ （130.13± 3.22） , （ 129.22 ± 5.02） ]had no difference compared with the UHMWPE group[ （ 149.22 ± 6.09 }, （ 140.52 ± 2.68 } ] （P 〉 0.05） ,there was no difference between the FYY720 group[ （68.24 ± 2.83）, （82.88 ± 5.34） 3and the blank groul）[ （69.51 ± 2.31 ）, （74.15 ± 4.64） { P 〉 0. 05） ;but the cytokine（ IL- 6 and TNF- α） concentrations in the UHMWPE groups were higher than the groups that without treatment UHMWPE （P 〈 0.05）. （1）Scanning electron microscopy showed that the area of resorption of the bone slices in the UHMWPE and FRY720 group[ （ 10.21 ~ 1.78） % was lower than the UHMWPE group[ {17. 79 ± 2.56） %33,the FTY720 group[ （1.82 ± 1.37）% was lower thau the blank groups[ （6.27 ± 2.11 }% 3;and the area of resorption of the bone slices in the UHMWPE groups were higher than the groups that without treatment UHMWPE （P 〈 0. 05 ）. （（~）RT- PCR results showed that osteeclasts - associated TRAP mRNA in the UHMWPE and FI＇Y720 group （ 0.53 -± 0.04 ） was lower than the UHMWPE group （0.74 ±0. 05 ）, the FTY720 group （0.22 ± 0.03 ） was lower than the blank group （0.38 ± 0.04） ;and osteeclasts- associated TRAP mRNA in the UHMWPE groups were higher than the groups that without treatment UHMWPE（ P 〈 0.05 ）. Couclusion： FTY720 could inhibit the bone destruction that caused by the UHMWPE in mousc air pouch mode.
出处
《激光杂志》
CAS
CSCD
北大核心
2012年第3期76-78,共3页
Laser Journal
基金
国家自然科学基金(NSFC
81071490)
