摘要
目的观察垂体腺苷酸环化酶激活肽（pituitaryadenylatecyclaseactivitingpolypeptide，PACAP）对大鼠刨伤性脑损伤（traumaticbraininjury，TBI）的影响及对血液和脾脏CD4＋、CD8＋T细胞数量的影响。方法所有实验均在在江苏省麻醉学重点实验室内完成。将雄性sD大鼠随机（随机数字法）分为假手术组（n=6）、生理盐水＋TBI组（rt=6）、PACAP＋TBI组（n=6），采用大鼠创伤性脑损伤模型，于创伤前20min侧脑室微量注射PACAP1μg／5μl，创伤后24h取材，采用HE染色方法观察脑组织损伤程度，采用流式细胞术检测各组大鼠血液及脾脏中CD4＋、CD8＋T细胞的数量。实验结果采用完全随机设计方差分析法。结果与假手术组比较，TBI大鼠创伤周围皮层神经元水肿、坏死，海马神经元排列紊乱，血液和脾脏CD4＋T细胞的数量减少（P=0．000，P=0．005），CD8＋T细胞的数量增加（P=0．01）；侧脑室内微量注射PACAP后，能明显减轻TBI大鼠皮层和海马的神经元损伤，增加血液和脾脏CD4＋T细胞的数量（P=0．019，P=0．839），降低CD8＋T细胞的数量。结论侧脑室微量注射PACAP能减轻大鼠创伤性脑损伤，可能与其对T细胞影响有关。
Objective To observe the effect of pituitary adenylate cyclase activiting polypeptide （PACAP） on traumatic brain injury （TBI） and on theCD4 ＋/CD8 ＋ T cell number in blood and spleen of rats. Methods The male SD rats were randomly （random number） divided into sham operation group （ n = 6）, normal saline ＋ TBI group （ n = 6） and PACAP ＋ TBI group （ n = 6）. Right parietal cortical contusion was produced by a weight-dropping method. PACAP was administered intra-cerebroventricularly in a dose of 1 μg/5 μl 20 min before TBI. Brain tissue samples were taken 24 h after trauma. The injured brain tissue of rats was examined by HE stain. The numbers of CD4＋/CD8＋ T cells in blood and spleen were deteced with flow cytometry. Results Edema, hemorrhage, inflammatory cell infiltration, swollen, degenerated neurons and neurons arrayed disorderly around the injured cortex in hippocampus were found under light microscope. The average numbers of CD4 ＋ T lymphocytes counts in blood and spleen were lower （ P = 0. 000, P = 0. 005 ）, and number of CD8 ＋ T lymphocytes was higher （ P = 0.01 ） in TBI rats group than those in the sham operation group. Micro-injection of PACAP into eerebroventricular attenuated the injury, significantly increased the number of CD4 ＋ T lymphocytes in blood and spleen （ P = 0. 019, P = 0. 839）, and decreased the number of CD8 ＋ T lymphocytes. Conclusions Pretreatment with PACAP may protect against TBI via influencing periphery T cellular immune function.
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2012年第6期572-576,共5页
Chinese Journal of Emergency Medicine
基金
南京军区创新项目（09MA036）
江苏省教育厅自然科学基金（09KJD310009）
