期刊文献+

靶向VEGF的不对称siRNA抑制乳腺癌MCF-7细胞的增殖 被引量:1

Asymmetric siRNA targeting VEGF inhibits proliferation of breast cancer MCF-7 cells
原文传递
导出
摘要 目的:探讨靶向血管内皮生长因子(vascular endothelial growth factor,VEGF)基因的siRNA对乳腺癌MCF-7细胞的抑制效果。方法:设计靶向VEGF的4种小干扰RNA(small interfering RNA,siRNA),包括对称siRNA(siRNA21/21,siRNA23/23)与不对称siRNA(asymmetric siRNA,aiRNA;aiRNA21/23,aiRNA19/21)。siRNA转染入MCF-7细胞后,MTT及流式细胞术检测MCF-7细胞增殖及凋亡情况,RT-PCR、ELISA法检测MCF-7细胞中VEGF基因和蛋白的表达。结果:与对照组相比,aiRNA21/23、siRNA23/23、aiRNA19/21、siRNA21/21这4种siRNA都能有效抑制VEGF mRNA的表达[(71.4±5.01)%、(40.0±3.11)%、(37.2±2.79)%、(11.1±0.99)%vs(2.4±0.11)%,P<0.01],且抑制MCF-7细胞的增殖[(44.7±5.38)%、(38.5±5.67)%、(33.6±2.18)%、(33.1±3.18)%vs(2.2±0.28)%,P<0.01],其中以不对称aiRNA21/23的抑制效果最明显。与对照组比较,aiRNA21/23显著促进MCF-7细胞的凋亡[(49.9±4.02)%vs(4.7±0.91)%,P<0.01]。结论:靶向VEGF基因的siRNA可抑制MCF-7细胞的增殖、促进细胞凋亡,尤以不对称siRNA的效果最明显。 Objective: To investigate the inhibitiory effect of siRNA targeting vascular endothelial growth factor(VEGF) gene on proliferation of breast cancer MCF-7 cells.Methods: Small interfering RNA(siRNA) targeting VEGF,including 2 siRNAs(siRNA21 /21,siRNA23 /23) and 2 asymmetric siRNAs(aiRNA)(aiRNA19 /21,aiRNA21 /23) were designed and synthesized.The siRNA was transfected into MCF-7 cells,and cell proliferation was detected by MTT method,and the apoptosis was analyzed by flow cytometry.VEGF protein and mRNA expression levels of MCF-7 cells were detected by ELISA and RT-PCR.Results: Compared with the control group,the 4 siRNAs effectively inhibited VEGF mRNA expression([71.4 ±5.01]%,[40.0 ±3.11]%,[37.2 ±2.79]%,[11.1 ±0.99]% vs[2.4 ±0.11]%,P 0.05),and the proliferation of MCF-7 cells([44.7 ± 5.38]%,[38.5 ± 5.67]%,[33.6 ± 2.18]%,[33.1 ± 3.18]%,[2.2 ± 0.28]%,P 0.05),with aiRNA21 /23 of the best RNA interference effect.Compared with the control group,the apoptotic rate was significantly increased([49.9 ± 4.02]% vs [4.7 ± 0.91]%,P 0.05).Conclusion: siRNA targeting VEGF can inhibit proliferation and induce apoptosis of MCF-7 cells,with asymmetric siRNA showing best effects.
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2012年第3期252-256,共5页 Chinese Journal of Cancer Biotherapy
基金 国家重大新药创制项目资助(No.2009ZX09103-635)~~
关键词 乳腺癌 MCF-7细胞 RNA干扰 不对称siRNA 血管内皮生长因子 增殖 凋亡 breast cancer MCF-7 cell RNA interference asymmetric siRNA vascular endothelial growth factor proliferation apoptosis
  • 相关文献

参考文献18

二级参考文献126

共引文献73

同被引文献23

  • 1Holstege H, Joosse SA, van Oostrom CT, et al. High incidence of protein- truncating TP53 mutations in BRCAI- related breast cancer [J]. Cancer Res, 2009,69 (8) :3625-3633.
  • 2LiC, CaoS, Liu Zet, et al. RNAi- mediated downregulation of uPAR synergizes with targeting of HER2 through the ERK pathway in breast cancer cells [J]. Cancer, 2010, 127 (7) : 1507-1516.
  • 3Singh R, Saini N. Downregulation of BCL2 by miRNAs augments druginduced apoptosis a combined computational and experimental approach [J]. J Cell Sci, 2011,125(6) : 1568-1578.
  • 4张哲,单保恩.IL-27基因修饰人树突状细胞(DC)疫苗(IL-27/DC)的抗肿瘤作用研究[C],河北省免疫学会第六次免疫学大会.
  • 5Van Pham P, Vu NB, Duong TT, et al. Suppression of human breast tumors in NOD/SCID mice by CD44 shRNA gene therapy combined with doxorubicin treatment [J ]. Onco Targets Ther, 2012, 5 : 77-84.
  • 6Liu C, Zhang N. Nanoparticles in gene therapy principles, prospects, and challenges [J]. Prog Mol Biol Transl Sci, 2011,104 : 509-562.
  • 7周昌华,彭晓东,吴静,张平,赵宗蓉,魏大鹏,章崇杰.质粒介导靶向c-myc的siRNA对MCF-7乳腺癌细胞增殖的影响[J].四川大学学报(医学版),2008,39(3):373-377. 被引量:9
  • 8王尧,郑启昌,胡文君,胡青钢,夏耘,张景辉.线粒体融合素基因-2对肝癌细胞凋亡的影响及其机制[J].中国普通外科杂志,2008,17(7):673-677. 被引量:6
  • 9朱财林,李南林,王廷.转染NDRG2基因抑制乳腺癌细胞增殖[J].基础医学与临床,2010,30(1):37-41. 被引量:3
  • 10李祥勇,周克元,林观平.抑癌基因PTEN对乳腺癌细胞生长增殖的影响[J].海南医学,2010,21(3):8-11. 被引量:12

引证文献1

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部