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携带癌胚抗原基因重组腺相关病毒感染DC诱导CTL活化 被引量：3

Activation of CTL induced by DC transfected with recombinant adeno-associated virus containing carcinoembryonic antigen gene
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摘要 目的:探讨携带癌胚抗原(carcinoembryonic antigen,CEA)基因重组腺相关病毒rAAV/CEA感染直肠癌患者的DC诱导CTL对肿瘤的杀伤作用。方法:抽取直肠癌患者的外周血培养DC细胞,用rAAV/CEA感染DC,DC成熟后与淋巴细胞混合诱导产生CTL,用流式细胞仪分别检测感染和未感染病毒的DC的表型及其诱导的CTL杀伤活性。结果:实验组DC的CD40、CD80、CD86表达显著增高[(81.95±1.45)%vs(72.27±1.59)%,(80.10±1.03)%vs(70.59±1.04)%,(58.93±1.84)%vs(48.66±1.97)%;均P<0.05]。实验组DC中CEA的表达率显著增高[(59.04±1.68)%vs(46.03±1.23)%;均P<0.05]。在效靶比为12.5∶1、25∶1、50∶1、100∶1时,实验组CTL对直肠癌LoVo细胞的杀伤率明显提高[(12.65±0.30)%vs(6.59±0.21)%,(25.77±2.53)%vs(8.35±1.12)%,(43.36±0.83)%vs(12.79±0.23)%,(61.03±1.83)%vs(23.35±0.69)%;均P<0.05],并且随着效应细胞比例的增加,杀伤率也逐渐增加。结论:携带CEA重组腺相关病毒感染DC激活CTL对肿瘤细胞能产生高效的杀伤作用。
作者 朱成英 董小林 韩亚萍 张俊萍
机构地区 山西医科大学研究生学院 山西医学科学院 山西大医院生物治疗科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2012年第3期313-316,共4页 Chinese Journal of Cancer Biotherapy
基金 山西省青年科技研究基金资助(No.2010021035-6)~~
关键词 树突状细胞 癌胚抗原基因 大肠癌 LOVO细胞
分类号 R735.3 [医药卫生—肿瘤] R730.54 [医药卫生—肿瘤]
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共引文献13

同被引文献60

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中国肿瘤生物治疗杂志
2012年 第3期

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