摘要
以刺五加愈伤组织为试材,研究了器官发生途径和体细胞胚胎发生途径获得再生植株的发生机制。结果表明:移栽成活率分别达到47%和86%;刺五加不定芽诱导最适培养基组成为:MS+NAA 0.2 mg/L+BA 3.0 mg/L;体胚诱导的最适培养基组成为:MS+蔗糖20g/L+葡萄糖20g/L;细胞学观察发现刺五加不定芽大多发生在愈伤组织表面;刺五加体胚发生与合子胚发育过程相似,发生途径为单细胞起源、内部发生。
Using Acanthopanax senticosus calli as materials,the regeneration plants through organogenesis and somatic embryogenesis were got.The results showed that the transplanting survival rate was 47%（organogenesis） and 86% （somatic embryogenesis）,respectively.Best combination for adventitious bud was MS medium supplemented with 0.2 mg/L NAA and 3.0 mg/L BA;while for somatic embryogenesis,MS medium supplemented with 20 g/L sucrose, 20 g/L glucose without hormonal was better than others.Histological observation showed that buds mostly formed on the surface of the callus,and somatic embryogenesis was similar to the process of zygotic embryogenesis,and it could be induced from internal callus and originated single cell.
出处
《北方园艺》
CAS
北大核心
2012年第12期120-124,共5页
Northern Horticulture
基金
吉林省科技厅资助项目(20080715)
关键词
刺五加
器官发生
体细胞胚胎发生
Acanthopanax senticosus
organogenesis
somatic embryogenesis
