摘要
目的探讨组织间植入125I粒子与5-FU对H22肝癌细胞生长及病理变化。方法 40只昆明小鼠皮下移植H22肝癌细胞建瘤模型,荷瘤小鼠随机分为4组,每组10只。A组为125I粒子联合5-FU组;B组为125I粒子组;C组为5-FU组;D组为空白对照组。连续观察28d小鼠存活情况,测量肿瘤体积大小,进行常规病理学及电镜检查。结果观察28d后,各治疗组和对照组小鼠的平均肿瘤体积分别为A组(1.38±0.11)cm3、B组(2.11±0.19)cm3、C组(2.79±0.23)cm3、D组(4.34±0.29)cm3,各组间肿瘤体积差异有统计学意义(P<0.05)。A、B、C3个治疗组间荷瘤小鼠的体积抑瘤率分别为68.21%、51.38%和35.71%。镜下观察B、C两组分别可见散落的坏死癌细胞,周围组织有大量淋巴细胞浸润;A组以癌细胞坏死为特征;电镜下B、C两组的肝癌细胞核膜完整,核仁浓缩或碎裂,染色质密集,有多个细胞质突起,出现染色质溶解消失,凋亡小体形成,其间有较多凋亡的癌细胞,并有多个凋亡细胞聚集;而125I联合5-FU组以坏死或不完全坏死的癌细胞为主,其间也有凋亡的癌细胞。结论肿瘤组织间植入125I粒子可促进H22肝癌细胞凋亡,125I粒子联合5-FU组织间化疗可进一步促使肿瘤细胞变性、坏死和凋亡。两者联合应用具有协同、增效的作用,疗效确切,为治疗肝癌提供了新的选择。
Objective To investigate the effects of a combination of interstitial implantation of an 125I seed with 5-FU on growth and pathological changes of hepatocarcinoma cell line H22. Methods A total of 40 KM mice were transplanted subcutaneously with H22 cells.Tumor-bearing mice were then divided randomly into 4 groups（each n = 10）.An 125I seed and 5-FU were embedded into the tumor of each mouse in group A;mice in groups B and C were treated,respectively, with an 125I seed or 5-FU;mice in group D did not receive any treatment.All animals were sacrificed 28 days later;survival rate and tumor size were measured,and tissue samples were analyzed by routine histopathology and electron microscopy. Results Mean tumor volumes in each group were as follows：group A,1.38±0.11 cm3;group B,2.11±0.19 cm3;group C,2.79±0.23 cm3;group D,4.34±0.29 cm3.Volumes were significantly different among the groups （P〈0.01）. The extent of tumor volume inhibition was 68.21% in group A, 51.38% in group B and 35.71% in group C. Histopathological observation showed that the major change in group A was cell necrosis, while samples from groups B and C showed scattered necrotic cancer cells,with a large number of lymphocytes around. Electron microscopy of group A samples showed complete or incomplete necrosis and sometimes apoptosis of cancer cells, while samples from groups B and C showed most tumor cells to have an integrated nuclear membrane,dense chromatin and numerous cytoplasmic protuberances or chromatolysis and apoptotic bodies.Samples from these two groups also showed numerous aggregated apoptotic cancer cells. Conclusion 125I seed interstitial implantation can promote apoptosis in H22 cells.Use of an 125I seed combined with 5-FU interstitial chemotherapy can further promote tumor cell degeneration, necrosis and apoptosis.The combination of 125I and 5-FU has synergistic effects,providing a new option for treating liver cancer.
出处
《中国癌症防治杂志》
CAS
2012年第2期145-149,共5页
CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT
基金
广西自然科学基金资助项目(桂科攻10124001A-3)
