摘要
目的观察比较聚蛋白多糖酶和基质金属蛋白酶MMP-1、3在骨关节炎(OA)不同时期关节液中的表达及作用。方法选择144例膝关节OA患者,软骨退变根据MRI Recht分级标准和关节镜下Outerbridge分级标准进行分级,并根据分级结果分为早、中、晚期3组。采用酶联免疫吸附法及蛋白免疫印迹法分别对3组关节液ADAMTS-4、5和MMP-1、3及其降解产物ARGxx、FFGxx的表达水平进行测定及比较。结果 ADAMTS-4、5在早期和晚期OA关节液中表达明显升高,且ADAMTS-4在早期OA组表达显著高于晚期OA组(P<0.01);与中期OA组相比,MMP-1在晚期OA关节液中高表达,而MMP-3在早期OA关节液中高表达(P<0.05);ARGxx在早期OA关节液中显著高表达(P<0.01),而FFGxx则主要表现在晚期OA关节液中(P<0.01);3组OA关节液中ADAMTS-5、MMP-3、ARGxx的表达水平均分别高于相应各组关节液中ADAMTS-4、MMP-1、FFGxx的表达(P<0.01)。ADAMTS-4和ADAMTS-5的表达均与ARGxx呈正相关(P<0.01),但ADAMTS-4与ARGxx的相关性(r=0.530)高于ADAMTS-5(r=0.258)。蛋白免疫印迹分析结果显示,ADAMTS-4在早期OA组高表达(P<0.05),ADAMTS-5在早期和晚期OA组高表达(P<0.05);MMP-1及FFGxx在晚期OA组高表达(P<0.05);MMP-3在早期OA组的表达量高于中期OA组(P<0.05);ARGxx在早、晚期OA组的表达量显著高于中期OA组(P<0.05)。结论聚蛋白多糖酶在骨关节炎早期和晚期软骨破坏中均起作用,而基质金属蛋白酶主要在晚期起作用。
Objective To compare the different effects of aggrecanases and MMPs by detecting the expression of AD- AMTS-4, ADAMTS-5, MMP-1, MMP-3 proteins in the synovial fluid （SF） of patients with different stages of osteo- arthritis （OA）. Methods A total of 144 patients with knee OA were studied and the patients were divided into early-, middle- and late-stage OA groups according to the degree of knee articular cartilage degeneration which was assessed by Recht＇s grading standard of MRI image and Outerbridge grading standard under arthroscope. Expressions of ADAMTS- 4, ADAMTS-5, MMP-1, MMP-3, ARGxx and FFGxx in the SF were investigated and compared by enzyme-linkedimmunosorbent assay and Western blot. Results Both ADAMTS-4 and ADAMTS-5 had higher expressions in the ear- ly- and late-stage OA groups than those in the middle-stage group, while the expression of ADAMTS-4 in the early- stage OA group was significantly higher than that in the late-stage OA group（P 〈0.01 ）. Compared with the middle- stage OA group, MMP-1 had higher expression in the late-stage OA group and MMP-3 had higher expression in the ear- ly-stage OA group （ P 〈 0.05 ）. ARGxx had a significantly higher level in the early-stage OA group （ P 〈 0.01 ）. Con- versely, FFGxx had higher expression in the late-stage group than those in the early- and middle-stage groups （ P 〈 0.01 ）. The exoression levels of ADAMTS-5, MMP-3 and ARGxx in the three groups were significantly higher thanthose of ADAMTS-4, MMP-1 and FFGxx respectively （ P 〈 0.01 ）. Both ADAMTS-4 and -5 expressions were positive- ly correlated with ARGxx （P 〈0.01 ）, and the expression of ADAMTS-4 was more closely related to it （ r =0.530 vs r = 0. 285 ）. Western blot analysis revealed the expression of ADAMTS-4 significantly increased in the early-stage group （ P 〈0.05 ）, while ADAMTS-5 had higher expression in both early-stage and late-stage groups （ P 〈 0.05 ）. MMP-1 and FFGxx had higher expression in the late-stage group （ P 〈 0.05 ）, while MMP-3 was higher in the early-stage group than in the middle-stage group （ P 〈 0.05 ）. Besides, the expressions of ARGxx in early- and late-stage OA groups were significantly higher than that in the middle-stage group （ P 〈 0.05 ）. Conclusion Aggrecanases are primarily involved in the cartilage destruction in the early and late stages of OA, however, matrix metalloproteinases mainly performed in the late stage of OA in human.
出处
《山东大学学报（医学版）》
CAS
北大核心
2012年第7期87-91,共5页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金(Y2007C132)
