摘要
将单增李斯特菌的多克隆抗体和羊抗鼠IgG(二抗)喷涂于硝酸纤维素膜(NC膜)上分别作为检测线C和质控线T。将单增李斯特菌的单克隆抗体与磁性纳米材料进行化学偶联构建磁性纳米探针,建立双抗夹心模式的检测试纸条。通过在层析体系中分别添加表面活性剂、盐离子、杂蛋白和糖类,探讨其对层析的影响,以提高检测的准确性和灵敏度。研究发现,在层析体系中添加一定浓度的Tween-20、KCl、BSA和葡聚糖,可以实现单增李斯特菌的快速检测。本文研究对今后研发类似磁性试纸条检测技术具有重要参考价值。
Anti-Listeria monocytogenes polyclonal antibody and goat anti-mouse IgG （bi-antibody） were sprayed on the nitrocellulose membrane respectively as test line T and the quality control line C. A double-antibody sandwich mode determination test paper was constructed by using anti-L, monocytogenes monoclonal antibody chemically coupled with magnetic nano-material as nano probe. By means of adding respective surfactants, salts ion, sundry protein, and carbohydrate to study their impact on chromatography system, in order to improve the accuracy and sensitivity of the test and determination. The study was found that certain concentration of Tween-20, KCl, BSA and dextran could realize rapid detection of L. monocytogenes. This study has important reference value to research and develop the similar magnetic test paper determination technology in the coming years.
出处
《微生物学杂志》
CAS
CSCD
2012年第3期37-42,共6页
Journal of Microbiology
基金
上海市科技兴农重点攻关项目(沪农科攻字(2010)第2-6号)
上海市教委科研创新项目(09YZ274)
上海市科学技术委员会部分地方院校能力建设项目(11310501100)
上海市科委工程中心建设(11DZ2280300)
关键词
免疫层析
单增李斯特菌
表面活性剂
盐离子
杂蛋白
糖类
immune chromatography
Listeria monocytogenes
surfactant
salt ions
sundry protein
carbohydrate
