摘要
目的:应用微弧氧化法对纯钛进行表面改性,形成含不同浓度钙离子、锌离子的涂层,探讨涂层对变形链球菌黏附和形态的影响。方法:用微弧氧化法在钛表面制备含不同浓度钙离子、锌离子涂层的5组样品,即低钙组(L-Ca)、高钙组(H-Ca)、低锌组(L-Zn)、中锌组(M-Zn)和高锌组(H-Zn),以未涂层机械抛光组(CP)作为对照组;用贴膜法评价6组钛样品对变形链球菌抗菌性能的影响;以扫描电镜(SEM)观察细菌在样品表面黏附数量和菌体形态的变化。采用SPSS 13.0软件包对数据进行统计学分析。结果:贴膜法实验表明,与CP组相比,L-Ca、H-Ca组抗菌性能无显著差异(P>0.05),含锌组抗菌效果显著(P<0.01),H-Zn组抗菌性显著高于L-Zn组(P<0.05)。扫描电镜观察,含锌组可影响细菌的黏附数量和菌体形态,且与锌的含量有关。结论:微弧氧化法制备的含锌离子涂层可有效抑制变形链球菌的黏附并影响其菌体形态,其具有的抗菌性能随锌含量的升高而增强。
PURPOSE： To prepare Ca and Zn-containing coatings using micro-arc oxidation on pure titanium surface and to investigate the coatings effect on S.mutans adhesion and morphology. METHODS： Five groups including low Ca- containing（L-Ca）,high Ca-containing（H-Ca）,low Zn-containing（L-Zn）,medium Zn-containing（M-Zn）,high Zn-containing （H-Zn） were prepared with micro-arc oxidation on titanium surface and machined commercial pure titanium（CP） was used as control group.Antibacterial properties of S.mutans on samples surface were appraised by the paster method and the change of bacteria was observed by SEM. SPSS 13.0 software package was used for all statistical analysis. RESULTS： The results showed that L-Ca and H-Ca groups had no significant difference in antibacterial property （P〉0.05）;Zn- containing groups had distinct antibacterial effect （P〈0.01） compared with CP group,and there was significant difference between L-Zn and H-Zn group （P〈0.05）.Meanwhile,Zn-containing groups could affect adhesion and morphology of S.mutans by SEM. CONCLUSIONS： Zn-containing coating prepared with micro-arc oxidation can effectively influence S.mutans adhesion and morphology, which shows better antibacterial effect with the increase of zinc content.
出处
《上海口腔医学》
CAS
CSCD
北大核心
2012年第3期266-269,共4页
Shanghai Journal of Stomatology
基金
辽宁省自然科学基金(20092093)
辽宁省教育厅资助项目(2004F008)
沈阳市科技计划项目(F11-264-1-25)~~
关键词
钛
微弧氧化
抗菌性
锌离子
钙离子
Titanium
Micro-arc oxidation
Antibacterial
Zinc ion
Calcium ion
