摘要
谷胱甘肽S-转移酶（GSTs）是一组多功能Ⅱ相药物代谢酶,能够清除外源性的亲电子化合物,包括致癌物、氧化应激产物和化疗药物等。己有研究表明,GSTs的过表达与肿瘤细胞化疗耐药性的产生存在着密切的关系,某些肿瘤耐药细胞株内GSTs含量要比敏感株高出许多倍,肿瘤细胞可通过表达GSTs而保护自身免受化疗药物的损害,
This study is to investigate the effects of ceramide on GSTA1 expression in Caco-2 cells. After being exposed to ceramide for a fixed time, GSTA1 protein expression was detected by Western blotting analysis; GSTA1 mRNA expression was detected by real time PCR; dual luciferase assay was used to analyze GSTA1 transcriptional activity and GSTA1 activity was determined toward androstanedione （AD） as substrate. The data showed that ceramide can significantly induce the expression of protein and GSTA1 mRNA, and increase transcriptional activity and enzyme activity of GSTA 1. The results demonstrated that ceramide may increase resistance to chemotherapeutics in Caco-2 cells by up-regulating the expression of GSTA1.
出处
《药学学报》
CAS
CSCD
北大核心
2012年第7期962-965,共4页
Acta Pharmaceutica Sinica
基金
加拿大自然科学研究基金资助项目(CIHR)
国家公派专项研究生奖学金项目(2009661006)
