摘要
目的建立测定抑毒调肝合剂中丹参素钠含量的反相高效液相色谱法。方法色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈-甲醇-水-冰醋酸(0.5∶8∶92∶1),流速1.0 mL/min,检测波长280 nm。结果丹参素钠质量浓度在32.52～162.60μg/mL范围内与峰面积线性关系良好(r=0.991 4),平均回收率为97.96%,RSD为0.88%(n=9)。结论该方法简便快速、准确、重复性好,可用于制剂中丹参素钠的含量测定。
Objective To establish an RP- HPLC method for the determination of sodium danshensu in Yidutiaogan Mixture. Methods The analysis was carried out at 30℃ on a Hypersil C18 column （250 min×4.6 mm,5 um）. The mobile phase consisted of acetonitrile-methanol-water-acetic acid（0. 5：8：92：1） and flow rate of 1.0 mL/min. The detection wavelength was 280 rim. Results The calibration curve of sodium danshensu was linear over the range of 32.52- 162.60 ug/mL（ r =0. 991 4）. The average recovery was 97.96%, RSD = 0. 88% （n = 9）. Conclusion This method is simple,accurate,reproducible and applicable for the content determination of sodium danshensu in Yidutiaogan Mixture.
出处
《中国药业》
CAS
2012年第11期29-30,共2页
China Pharmaceuticals
基金
泸州医学院青年基金项目
项目编号:川教函[2010]34
