摘要
目的观察HIF-1α和VEGF在骨肉瘤组织中的表达，并分析两者相关性，同时探讨其在骨肉瘤血管形成中所起的作用。方法应用免疫组织化学SP法，检测35例骨肉瘤、14例骨软骨瘤中HIF-1α和VEGF的表达情况，同时用CD34标记血管内皮细胞，计算微血管密度（MVD）。结果35例骨肉瘤组织中，HIF-1α蛋白的阳性表达率为34．3％（12／35），与对照组相比差别有非常统计学意义（P〈O．01）；VEGF的阳性表达率为82．9％（29／35），亦显著高于对照组（P〈0．01）；HIF-1α和VEGF的表达同时呈阳性，13例（37．1％）同时呈阴性，两者表达显著相关（r=0．589，P=0．000），且与癌组织分期显著相关（P〈0．05）；HIF-1α和VEGF均阳性的骨肉瘤组织中MVD值高于两者均为阴性者，两者相比较差异有非常统计学意义（P〈0．01）。结论HIF-1α和VEGF在骨肉瘤组织中均为高表达，提示它们可能对骨肉瘤新生血管生成中具有协同作用，在骨肉瘤的发生、发展起到重要作用。
Objective To investigate the role of HIF-1α and VEGF in angiogenesis of osteosarcoma by analyze their expression. Methods The expression of HIF-la and VEGF specimens of 35 osteosarcoma and 14 osteochondroma tissues was evaluated by immunohistoehemistry. MVD was calculated by using CD34 as the specific marker of microvessel endothelium. Results The positive rat（34.3%, 82.9 % ） of HIF-1α and VEGF in osteosarcoma were significantly higher than those in osteochondroma tissues[P〈0.01 ）. The expressions of HIF-1α and VEGF in osteo- sarcoma were positively correlated（P〈0.05）. The expression of HIF-1α and VEGF were correlated with Staging （P〈0.05）. The MVD was higher in the positive group of HIF-1α and VEGF than their negative group（ P〈0.01）. Conclusions The positive expression of HIF-1α and VEGF are related with osteosarcoma microvascular generate. It seems that HIF-1αand VEGF contribute to development of osteosarcoma.
出处
《中国误诊学杂志》
CAS
2012年第14期3525-3527,共3页
Chinese Journal of Misdiagnostics
关键词
骨肉瘤/代谢
缺氧诱导因子1
α亚基/代谢
血管内皮生长因子A/代谢
新生血管化
病理性
Osteosarcoma/metabolism
Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
Vascular Endothelial Growth Factor A/metabolism
Neovascularization, Pathologic
