摘要
应用高效液相色谱-电喷雾/四极杆飞行时间串联质谱联用技术(HPLC-ESI/Q-TOF-MS/MS)分析了3种荷花(大红袍、洪湖红莲、红牡丹)的花色苷成分。首先以φHCl=0.1%的甲醇超声提取荷花中的花色苷,然后用XAD-7大孔树脂纯化,选用Amethyst C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(φC2HF3O2=0.05%)为流动相进行梯度洗脱,柱后流出液采用电喷雾四极杆飞行时间质谱(ESI/Q-TOF-MS/MS)进行检测,共分离鉴定出6种花色苷,并且运用pH示差法分别测定了3种荷花中总花色苷的含量,为开发利用这一丰富的自然资源提供一定的参考依据。
We employed HPLC-Electrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry （ HPLC-ESI/ Q-TOF-MS/MS） to analyze the anthocyanins in three species Nelumbo nucifera. The anthocyanins were extracted with methanol containing HCI （φFacl = 0. 1%） and then purified on an Amberlite XAD-7 column. We applied an Amethyst C18 column （250 mm ×4.6 mm, 5 μm） to HPLC analysis with water methanol-water （containing trifluoroacetic acid （φC1HF3O3 = 0.0.5% ） ） in gradient as mobile phase. The eluates were then detected by ESI/Q-TOF-MS/MS. We altogether identified six s.pecies anthocyanins from these Nelumbo nucifera. We further determined their contents by pH-differential spectrophotometry. It provides theoretical foundation for further development and utilization of Nelumbo nucifera resources.
出处
《山东科学》
CAS
2012年第3期66-70,共5页
Shandong Science
基金
作物生物学国家重点实验室开放课题(20082KF05)
山东省科技攻关项目(2010GSF10287)
关键词
高效液相色谱-四级杆飞行时间串联质谱
荷花
花色苷
HPLC-electrospray ionization/quadrupole-time of flight-mass/mass spectrometry （ HPLC-ESI/Q-TOF-MS/ MS）
Nelumbo nucifera
anthocyanins
