摘要
为了明确棉铃虫Helicoverpa armigera(Hübner)取食转双价基因(Bt+CpTI)棉叶以及取食一段时间转基因棉叶后,再取食常规棉叶,对棉铃虫取食量、体重增长量及中肠羧酸酯酶活性的影响,本研究分别用转双价基因棉叶和常规棉叶饲喂4龄棉铃虫幼虫5 d,比较考察了棉铃虫的取食量、体重增长量及中肠羧酸酯酶的活性;另外分别考察了棉铃虫取食转基因棉叶1 d及3 d后再取食常规棉叶,其中肠羧酸酯酶的变化。结果表明,持续取食常规棉花叶片5 d的棉铃虫,其中肠羧酸酯酶的活性持续升高;而持续取食转基因棉叶5 d的棉铃虫,其中肠羧酸酯酶的活性先升高后降低。取食转基因棉花叶片1 d后,取食常规棉叶的棉铃虫,其中肠羧酸酯酶的活性,随着换取常规棉叶时间的延长,活性逐渐降低;而棉铃虫在取食3 d转基因棉叶后再取食常规棉叶,其中肠羧酸酯酶活性却一直保持较高。可见,棉铃虫在取食转基因棉花后,其羧酸酯酶活性可以被诱导,这应与棉铃虫对转基因棉的抗性及防御性有一定关系。
In order to clarify changes in the carboxylesterase （ CarEs ） activity of Helicoverpa armigera （Htibner） feeding on transgenic cotton, and the recovery of carboxylesterase activity in H. armigera that fed on non-transgenic cotton after feeding on transgenic cotton for 1 to 3 days, transgenic and non transgenic cotton leaves were fed to some fourth-instar H. armigera larva for 5 days, after which some were fed with non-transgenic cotton leaves for a few days after feeding on transgenic cotton leaves for 1 day or 3 days. Midgut CarEs activity was examined every 24 hours. The results show that CarEs activity in H. armigera fed on non-transgenic cotton leaves increased over time, whereas that of H. armigera fed on transgenic cotton leaves first increased then decreased. The enzyme activity of cotton bollworms fed on non-transgenic cotton leaves after feeding on transgenic cotton leaves for one day decreased gradually over time, whereas that of those fed on non-transgenic cotton leaves after feeding on transgenie cotton leaves for three days remained higher than the control. These results indicate that CarEs activity in H. armigera can be induced by feeding on trangenetic cotton leaves and that this plays a role in the resistance of H. armigera to the CrylAc toxin.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2012年第4期847-852,共6页
Chinese Journal of Applied Entomology
基金
转基因生物新品种培育重大专项(2008ZX08012-04)
农业部行业专项农药高效安全科学施用技术(200903033-02)
