摘要
目的 观察微量元素硒 (Se)对大鼠肝细胞、储脂 (Ito)细胞过氧化脂质和细胞外基质 (ECM)产生的影响。方法 采用原位灌注方法分离大鼠肝细胞和 Ito细胞。结果 培养肝细胞、Ito细胞有一定水平的基础丙二醛(MDA)产生和 ECM分泌。Se可提高肝细胞、Ito细胞谷胱甘肽过氧化物酶 (GSH- Px)活性 ,抑制基础 MDA产生 ,减少 3H-脯氨酸 (3H- Pro)掺入、 型前胶原 (PC )分泌及 Ito细胞透明质酸 (HA)分泌。结论 Se可抑制肝细胞和 Ito细胞过氧化脂质产生和
Objective Study the inhibitory effects of selenium on the production of basic lipid peroxidation and the secretion of extracellular matrix(ECM) of hepatocytes and ito cells in vitro.Methods Rat hepatocytes and ito cells were isolated and cultured.Results The results showed that selenium could obviously lower the production of malondialdehyde(MDA) and raise the activity of glutathione peroxidase(GSH Px) and inhibit the secretion of precollagen type Ⅲ (PCⅢ) in hepatocytes and ito cells as well as inhibit the secretion of hyaluronic acid in ito cells,and that there was a negative correlation between the level of PCⅢ and the ratio of GSH Px/MDA in both cells and a negative correlation between the levels of hyaluronic acid and the ratio of GSH Px/MDA in ito cells.Conclusions These studies indicated that selenium could lower the production of lipid peroxidation and inhibit the secretion of ECMs by raising the activity of GSH Px of rat hepatocytes and ito cells. [
出处
《中国地方病学杂志》
CAS
CSCD
2000年第3期180-182,共3页
Chinese Jouranl of Endemiology
基金
吉林省科技发展基金 !(2 0 0 0 30 0
关键词
硒
肝细胞
储脂细胞
脂质过氧化
细胞外基质
Selenium
Hepatocyte
Ito cell
Lipid peroxidation
Extracellular matrix
