摘要
目的探讨乙肝病毒侵犯淋巴细胞对慢性乙肝患者免疫功能状态的影响。方法采用双抗体夹心法检测慢性乙型肝炎患者外周血单个核细胞（ＰＢＭＣ）培养上清液中细胞因子的水平， ＰＣＲ方法扩增乙肝病毒ＤＮＡ片段。血清中ＨＢＶ ＤＮＡ检测采用斑点杂交法。结果与正常对照组比较，慢性乙型肝炎患者的 ＩＦＮ－γ水平降低，但无显著差异性：而ＩＬ－４水平明显增高（Ｆ＝１７.４，Ｐ＜０．０１）。血清中ＨＢＶ ＤＮＡ水平与 ＰＢＭＣ培养上清液中的ＩＦＮ－γ水平呈负相关（ｒ_ｓ＝－０.５３，Ｐ＜０.０１）。６２.９％（３５／３６）的慢性乙肝患者外周血ＰＢＭＣ中检出ＨＢＶ ＤＮＡ，ＨＢＶ ＤＮＡ阳性组和阴性组的ＩＦＮ－γ水平具有显著差异（Ｆ＝１５.２，Ｐ＜０.０１）。结论（１）慢性乙肝患者的外周血以 Ｔｈ_２类细胞因子占优势。（２）乙肝病毒侵犯淋巴细胞可能影响其免疫功能的发挥。
Objective To explore the influence of HBV invasion into peripheral blood mononuclear cells (PBMC) on the immune response of patients with chronic hepatitis B. Methods The cytokine levels in the culture supernatant of PnMC from 56 patients with chronic hepatitis B were determined by ELISA, and plymerase chain reaction (PCR) method was employed to amplify the HBV DNA fragment Results The level of IFN-γin patients with hepatitis n was lower than that of the control, but the difference was not statistically significant in contrast, the level of IL-4 were significantly high., than that of the control (P<0.01 ) The serum level of HBV DNA were negatively correlated with that of IFN-γ in culture supernatants of PBMC. Thirty-five patients with HBV DNA positive in PBMC were identified from 56 patients with hepatitis B, and their INN-γ level proved to be significantly different Conclusions (1) Th2 cellmediated immune response is predominant in chronic hepatitis B, which is associated with the chronicity of HBV infection. (2) HBV invasion into PBMC may affect Th1 and Th2 cell-mediated immune response of the patients with chronic hepatitis B.
出处
《第一军医大学学报》
CSCD
2000年第2期117-119,共3页
Journal of First Military Medical University
