摘要
目的：研究来氟米特在新生ＳＤ大鼠皮肤匀浆中的代谢作用，为来氟米特经皮给药制剂的研究提供实验依据。方法：取新生 ＳＤ大鼠腹部皮肤于生理盐水中匀浆，制备高、中、低 ３组浓度来氟米特的皮肤匀浆液， ３２℃保温，适时取样，以ＨＰＬＣ法测定来氟米特和其活性代谢物Ａ７７ １７２６的浓度。结果：除对照组（不含皮肤匀浆）中来氟米特浓度保持不变外，其他各组中来氟米特浓度均随保温时间的延长而降低，活性代谢物Ａ７７ １７２６的浓度随保温时间的延长而增加。结论：来氟米特在新生ＳＤ大鼠皮肤匀浆上清中具有较明显的代谢作用。
Objective: To study the skin metabolism of leflunomide in neonatal SD rat skin homogenate. Methods: Neonatal SD rat abdominal skin was homogenized in normal saline, and 3 different concentrations of leflunomide was added into the skin homogenate solution. These samples were incubated at 32℃, the concentration was measured by HPLC. Results: The concentration of leflunomide declined and the concentration of active metabolite A77 1726 rose in the skin homogenate solution as a prolonging of time, while no appreciable change in the drug concentration in the normal saline was observed. Conclusion: The effect of skin metabolism of leflunomide in neonatal SD rat skin is obvious.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第4期378-380,共3页
Academic Journal of Second Military Medical University
基金
全军"九五"医学科研规划基金资助项目!(98M077)
关键词
来氟米特
皮肤代谢
高效液相色谱法
大鼠
leflunomide
skin metabolism
chromatography, high performance liquid
