摘要
目的：探讨移植骨髓问质干细胞（BMSCs）在损伤脊髓内分化为功能性神经元的可能性。方法：应用低温包埋免疫电镜技术观察迁移在损伤脊髓内1、3、5周后的移植BMSCs的超微结构，应用免疫荧光标记和激光共聚焦技术观察迁移存损伤脊髓内1、3、5周后的移植BMSCs表达胆碱乙酰基转移酶（CHAT）、谷氨酸脱羧酶（GAD）和突触素（synapsins）的情况。结果：移植1周后，迁移在损伤脊髓灰质内的BMSCs细胞器不发达，不具有神经元的超微结构特点。移植3周后，迁移在损伤脊髓灰质内后的BMSCs细胞器开始发达，具有神经元的超微结构特点。移植1周后，迁移在损伤脊髓灰质内后的BMSCs不表达ChAT、GAD和突触素。移植3周后，迁移在损伤脊髓灰质内的BMSCs开始表达ChAT、GAD和突触素。结论：移植BMSCs在损伤脊髓内可部分分化为功能性神经元。
Objective： To explore the possibility that implanted bone marrow mesenchymal stem cells （BMSCs） can differen tiate into neurons within injured spinal cord in rats. Methods： The BMSCs were implanted into the injured spinal cord of rats, and then underwent ultrastructural observation using immuno-electron microscopy, and immunohistochemical analysis against CHAT, GAD and synapsins on postsurgical week 1, 3 and 5. Results： One week after implantation, the BMSCs mi grated into the grey matter of the spinal cord of rats with undeveloped organelles. The BMSCs did not exhibit the ultraslrue tural feathers of neuron, and negative immunoreactivity against ChAT, GAD and synapsins. Three weeks later, the implan ted BMSCs within grey matter of the spinal cord of rats had developed organelles, ultrastructural feathers of neuron, and positive immunoreactivity against CHAT, GAD and synapsins. Conclusion： The implanted BMSCs within the injured spinal cord of rats can differentiate partly into functional neurons.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第3期282-285,335,共5页
Chinese Journal of Anatomy
基金
教育部博士学科点科研基金（2006E＋10）
福建医科大学教授学术发展基金（JS08004）
关键词
骨髓间质干细胞
体内分化
免疫电镜
免疫组织化学
脊髓
bone marrow mesenchymal stem cells
differentiation in vivo
irrurnuno-eleetron microscope
immunohistocbemi try
spinal cord
