摘要
报道了测定多糖中单糖组成的糖醇乙酸酯的毛细管气相色谱分析方法。使用OV -225毛细管气相色谱柱分离了11种单糖的糖醇乙酸酯衍生物,在0.2～1.68g/L质量浓度范围内,11种单糖定量校正曲线的线性关系良好。应用该法测定了胡麻多糖、当归多糖和海带多糖中单糖的组成。
Determination of monosaccharides in polysaccharides is reported by gas chromatography with OV-225 capillary column. Monosaccharides from the polysaccharides hydrolyzed in aqueous trifluoroacetic acid are reduced by NaBH4. Reduced monosaccharides are derivatized into sugar alcohol acetates with acetic anhydride pyridine before determination. The method was used to determine monosaccharides in polysaccharides from flak, angelica sinensis, and laminaria. The results showed that the monosaccharides in polysaccharide included rhamnose, arabinose, xylose, galactose and glucose for flax; rhamnose, arabinose, galactose and glucose for angelica sinensis; fucose, galactose, mannose, glucose and xylose for laminaria.
出处
《分析测试学报》
CAS
CSCD
2000年第3期71-73,共3页
Journal of Instrumental Analysis
关键词
多糖
气相色谱分析
单糖
Polysaccharide
Acetylation
Gas chromatography
Monosaccharide
