摘要
目的研究肾石通片的质量标准。方法增加薄层法鉴别木香、金钱草及RP-HPLC法测定丹参中丹酚酸B的含量。含量采用Waters C18色谱柱;流动相:甲醇-乙腈-甲酸-水(30∶10∶1∶59);流速:1mL·min-1;检测波长:286nm。结果鉴别试验:阴性对照均无干扰;含量测定:丹酚酸B在0.017～2.25μg范围内呈良好的线性关系(r=0.9999),平均加样回收率为98.9%(n=6)。结论所建立方法简单、可靠,可用于肾石通片的质量控制。
Objective To establish a method for the quality control of Shenshitong Tablets. Methods The TLC methods were employed for Radix Aucklandiae and Herba Lysimachiae. RP-HPLC method was established for the determination of salvianolic acid B performed on Waters C18 analytical column with methanol-acetonitrile-formic acid-water （30 ： 10 ： 1 ： 59） as the mobile phase at the flow rate of 1 mL- min^-1. The detection wavelength was set at 286nm. Results Identification test： there was no interference from negative control; content determination： a good linear relationship （r= 0. 9999） was observed in the range of 0. 017-2.25 μg for salvianolic acid B, and the average recovery （n=6） was 98. 9%. Conclusion The method is convenient, reliable and suitable for quality control of shengshitong Tablets.
出处
《中国药事》
CAS
2012年第6期556-559,共4页
Chinese Pharmaceutical Affairs
基金
国家药品标准提高行动计划(编号[2005]167)
