摘要
目的:建立虚寒胃痛颗粒质量标准。方法:采用TLC法鉴别黄芪、白芍、桂枝、甘草;采用HPLC-ELSD法测定虚寒胃痛颗粒中黄芪的活性成分黄芪甲苷的含量:使用岛津VP-ODS(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(35∶65)为流动相,流量1.0 mL·min-1。结果:TLC色谱中可检出黄芪、白芍、桂枝、甘草,阴性均无干扰;黄芪甲苷在0.4892～12.23μg范围内,线性关系良好,平均回收率96.2%(RSD=1.9%,n=6)。结论:该方法准确,重复性好,可用于虚寒胃痛颗粒的质量控制。
Objective： To establish a method for the quality standard of Xuhan Weitong Granules. Methods： Radix Astragali, Radix Paeonia Alba, Ramulus Cinnamomum and Radix et Rhizoma Glycyrrhizae were identified by TLC. The content of Astragaloside IV was determind by HPLC-ELSD. HPLC was carried on with a Shimadzu VP-ODS （4. 6 mm× 150 mm,5 μm） column, acetonitrile water（35 ：65 ） as the mobile phase at the flow rate of 1.0 mL min-1. Results： Radix Astragali, Radix Paeonia Alba, Ramulus Cinnamomum and Radix et Rhizoma Glycyrrhizae could be identified by TLC, and the blank test showed that other medicine herbs had not interfer- ence with the main one; Astragaloside IV1 showed a good linear relationship in the range of O. 489 2-12. 23 μg; the average recovery was 96. 2% （RSD = 1.9%, n = 6）. Conclusion： The method is accurate with a good reproducibility, and suitable for quality control of Xuhan Weitong Granules.
出处
《中国药品标准》
CAS
2012年第3期184-188,共5页
Drug Standards of China
