摘要
In this study, nano-capsules of lansiumamide B （NCLB） was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B （LB）. An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency （E, %） value. The optimized NCLB were spherical and uniform under transmission electron microscopy （TEM）. The mean particle size, zeta potential and E, were （38.50~0.64） nm, （-70.5~0.76） mV and （95.13± 1.16）%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 ofMeloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LCs0 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1 significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.
In this study, nano-capsules of lansiumamide B （NCLB） was prepared by the microemulsion polymerization method to improve the nematicidal efficacy of lansiumamide B （LB）. An optimal formulation was gained by orthogonal experiment design based on the encapsulation efficiency （E, %） value. The optimized NCLB were spherical and uniform under transmission electron microscopy （TEM）. The mean particle size, zeta potential and E, were （38.50~0.64） nm, （-70.5~0.76） mV and （95.13± 1.16）%, respectively. The release profile indicated that the accumulated release of LB in NCLB reached up to 82% within 96 h. Effects of NCLB against Bursaphelenehus xylophilus and J2 ofMeloidogyne incognita were reported in this paper. The nematicidal activity of NCLB has been remarkably increased, with LCs0 values of 2.1407 mg L-1 and 19.3608 mg L-1, respectively, at 24 h after treatment. The disease progression and the average number of root knots of Ipomoea aquatica were 1.50 and 7.25, respectively, in the treatment of NCLB, at concentration of 200 mg L-1 significantly lower than the treatment of LB and ethoprophos. Compared to control, the treatments of NCLB, LB and ethoprophos leaded the disease progression to drop 68.42, 36.84 and 26.32%, respectively, and caused the average number of root knots to fall 83.94, 78.03 and 63.66%. These results suggested that NCLB, as a novel nematicides formulation, performed more efficient and longer effective maintenance against plant parasitic nematodes.
基金
Guangzhou Science and Technology Plan Projects, Guangzhou, China(10C62101593)
