摘要
目的了解血清学定型为B(A)血型标本的分子基础。方法采用PCR-SSP方法检测以血清学方法定型为B(A)血型的4名献血者和8例送检患者标本的ABO基因型;对于有O等位基因的杂合子标本,使用特异性引物分别扩增O和B等位基因的7号外显子编码序列,并做测序分析。结果 12例采用血清学方法定为B(A)血型标本的ABO基因型分别为B/B型1例(8.33%),B/O1型4例(33.33%),B/O2型7例(58.33%);序列分析显示:B(A)02等位基因为5例(41.67%),B(A)04等位基因为7例(58.33%)。结论 B(A)04和B(A)02等位基因可能是我国北方汉族人群B(A)血型中主要的遗传类型。
Objective To study the molecular background of B （A） blood group identified by serological tests. Methods PCR-SSP technique and DNA sequencing analysis were performed by using specially designed primers. Results One ease of B/B ,4 cases of B/O1 and 7 cases of B/02 were found after genotyping on 12 unrelated B （A） phenotype samples. DNA sequencing analysis showed 7 cases of B（A）04 and 5 eases of B（A）02. Conclusion B（A）04 and B（A）02 should be a predominant alleles in Han population of Chinese northern.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2012年第5期429-431,共3页
Chinese Journal of Blood Transfusion
基金
北京市科技新星计划(20061333)
