摘要
目的探讨高压氧（HBO）预处理对皮瓣移植术后大鼠缺血再灌注损伤炎症反应的影响。方法56只SD大鼠按数字表法随机均分为7组：假手术组（SH组）、缺血再灌注1d组、3d组、5d组（IR1d组、3d组、5d组）、HBO预处理＋缺血再灌注1d组、3d组、5d组（HBO＋IR1d组、3d组、5d组）。建立腹部带蒂移植皮瓣动物模型，进行HBO预处理，应用ELISA法检测各组大鼠血清中性粒细胞弹性蛋白酶（NE）及高迁移率族蛋白B1（HMGBl）水平。结果IR1d组、3d组和5d组NE水平明显高于SH组（P〈0．01），HBO4-IR1d组NE水平明显低于IR1d组（P〈0．01），HBO＋IR3d组和5d组NE水平低于相应的IR组（P〈0．05）。IR1d组、3d组和5d组HMGBl水平明显高于SH组（P〈0．01）；HBO＋IR1d组、3d组HMGBl水平低于相应IR组（P〈0．05）；HBO＋IR5d组HMGBl水平明显低于相应IR组（P〈0．01）。结论HBO预处理可使皮瓣移植术后大鼠NE和HMGBl水平降低，减轻移植术后缺血再灌注损伤造成的炎症反应，提高皮瓣成活率。
Objective To investigate the effects of hyperbaric oxygen （HBO） preconditioning on inflammatory reaction induced by ischemia-reperfusion （IR） in rats following skin flap transplantation. Methods Fifty-six Sprague-Dawley rats were randomly divided into 7 groups ： the sham group, the ischemia-reperfusion （IR） day 1 group, the IR day 3 group, the IR day 5 group, the HBO preconditioning ＋ ischemia -reperfusion day 1 group, the HBO ＋ ischemia-reperfusion day 3 group, and the HBO ＋ ischemia-reperfusion day 5 group. The abdominal pedicle skin flap animal model was established and the animals received HBO preconditioning. The levels of neutrophil elastase （ NE ） and high mobility group box 1 （ HMGB1 ） of 7 animal groups were measured with ELLSA. Results The levels of NE in the IR day 1, day 3 and day 5 groups were obviously higher than those of the sham group. The NE level of the HBO preconditioning ＋ IR day 1 group was obviously lower than that of the IR group （P 〈0.01 ）, and the NE levels of the HBO preconditioning ＋ IR day 3 and clay 5 groups were lower than those of the IR group（ P 〈 0.05 ）. The levels of HMGB1 in the IR day 1, day 3 and day 5 groups were significantly higher than that of the sham group （P 〈0.01 ）. The HMGB1 levels of the HBO preconditioning ＋ IR day 1 and day 3 groups were lower than those of the IR group （P 〈0.05）. The HMGB1 level of the HBO preconditioning ＋ IR day 5 group was obviously lower than that of the IR group （ P 〈 0.01 ）. Conclusions Experiments showed that HBO preconditioning could decrease the levels of NE and HMGB1 inrats following skin flap transplantation, alleviate inflammatory reaction induced by ischemia-reperfusion following skin flap transplantation, and increase survival rate of the grafted skin flap.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
北大核心
2012年第3期176-179,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
关键词
高压氧预处理
皮瓣移植
弹性蛋白酶
高迁移率族蛋白B1
hyperbaric oxygen preconditioning
Skin flap transplantation
Neutrophil elastase
High mobility gooap box 1
