摘要
目的了解对氨基糖苷类抗生素耐药的铜绿假单胞菌氨基糖苷类修饰酶(AMEs)基因和甲酰化酶基因的存在情况。方法用聚合酶链反应(PCR)测定临床分离的170株对氨基糖苷类抗生素耐药的铜绿假单胞菌5种主要的AMEs基因和2种甲酰化酶基因。结果铜绿假单胞菌的AMEs基因携带率较高(94.71%),其修饰酶基因检出率分别为aac(3)-Ⅱ 77.6%、aac(6')-Ⅰ 41.2%、ant(3″)-Ⅰ 40.0%、aph(3')-Ⅵ 21.8%、aac(3)-Ⅰ 0.0%,同时携带2种或2种以上AMEs基因的菌株占63.53%,甲酰化酶基因检出率分别为rmtD 1.8%、rmtA 0.0%。结论铜绿假单胞菌中AMEs基因携带率较高,同一菌株携带多个不同的AMEs基因现象较多;甲酰化酶基因携带率极低。
Objective To study the existing situation of aminoglycoside modifying enzyme（AMEs）gene and methylation gene of aminoglycoside antibiotics resistant Pseudomonas aerugionsa.Methods One hundred and seventy strains of aminoglycoside antibiotics resistant Pseudomonas aerugionsa were separated,meanwhile,5 main kinds of AMEs gene and 2 methylation genes were detected by polymerase chain reaction（PCR）.Results The AMEs gene carrying rate of Pseudomonas aerugionsa was high（94.71%）,and the detection rates of its modification genes were aac（3）-Ⅱ 77.6%,aac（6′）-Ⅰ 41.2%,ant（3″）-Ⅰ 40.0%,aph（3′）-Ⅵ 21.8%,aac（3）-Ⅰ 0.0%,resectively.The isolates rate that simultaneously carrying two or more genetypes was 63.53%,and the detection rates of methylation genes were rmtD 1.8%,rmtA 0.0%.Conclusion The Pseudomonas aerugionsa that carrying the AMEs gene has a high level,and the genetype is multiple;but the carrying rate of methylation is very low.
出处
《新乡医学院学报》
CAS
2012年第7期502-504,共3页
Journal of Xinxiang Medical University
关键词
铜绿假单胞菌
氨基糖苷类修饰酶
甲酰化酶
基因型
耐药
Pseudomonas aeruginosa； aminoglycoside modifying enzyme； methylation enzyme； genotype； drug resistance；
