摘要
目的模拟牙周炎患者日常生活中的牙周干预措施,研究各种牙周干预措施对SD大鼠动脉粥样硬化(As)发生、发展的影响。方法 SD大鼠随机分为4组:正常对照组(A组)、As组(B组)、As合并牙周炎组(C组)、牙周炎组(D组),将C组根据牙周干预措施不同再分为不治疗组(C1组)、刮治组(C2组)、药物治疗组(C3组)和拔除患牙组(C4组)。对各组进行相应的建模处理,苏木精-伊红染色,光学显微镜下观察牙周组织、颈动脉血管壁组织的病理变化,酶联免疫吸附(ELISA)法检测血清超敏C反应蛋白(hsCRP)的含量。结果病理切片发现B组、C3组和D组颈动脉血管壁均可见大量泡沫细胞形成、聚集;C1组和C4组可见内膜下有钙盐沉积,中膜弹力纤维紊乱、破坏;C2组可见纤维帽的形成及斑块破裂。牙周干预处理后,所有建模组和干预处理组的血清hsCRP含量均较A组明显升高(P<0.05);C1组、C2组、C3组的hsCRP含量较B组明显升高(P<0.05);且C2组hsCRP的含量高于C1组,差异具有统计学意义(P<0.05)。结论对于SD大鼠,无论有无高脂状态,牙周炎均可引起或加重As的发生发展;而在高脂状态下,直接牙周干预都可能加重As病变,其中牙周直接刮治处理的影响在短期内可能会更严重,且hsCRP可能参与了As加重的病变过程。
Objective We established the periodontitis and atherosclerosis （As） model in rats, simulated with periodontal interventions （including no treatment, tooth extractions, systemic antibiotics, scaling and root planning） to explore the relationship between periodontal diseases and atherosclerosis. Methods The SD rats were seperated into 4 groups： Control group （A group）, As group （B group）, As＋periodontitis group （C group）, periodontitis group （D group）. As＋periodontitis groups were seperated in to C1 group （no treatment）, C2 group （scaling and root planning）, C3 group （systemic antibiotics treatment）, C4 group （tooth extractions）. The pathological lesion of carotid artery plaque was stained with hematoxylin and eosin. Serum hsCRP levels were evaluated before periodontal interventions and 1 weeks after interventions by ELISA analysis. All animals were scarificed after 14 weeks. Results Histologic sections revealed increased foam cells infiltration in group B （atherosclerosis）, group D （periodontitis） and group C3 （systemic antibiotics） ; In the group C1 （no treatment） and group C4 （dental extractions）, foam cells and inflammatory cells were evident. In group C2 （scaling and root planning）, fibrous cap of atherosclerotic plaque formed and even ruptured. After periodontal interventions, the serum hsCRP of group B and C was significantly higher than control group （P 〈 0.05） ; Compared with group B, the levelsof serum hsCRP of group C2 and C3 were significantly increased （P 〈 0.05）. The serum hsCRP of group C2 was significantly higher than the group C1 （P 〈 0.05）. Conclusions Periodontitis may accelerates the progression of atherosclerosis in rats. In rats with periodontitis associated with systemically cardiovascular diseases or systemic inflammation, direct periodontal interventions may accelerate carotid atherogenic plaque progression, especially scaling and root planing. Therefore, proper time of periodontal basic therapy on patients with cardiovascular diseases should be carefully considered.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2012年第3期28-33,共6页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
国家自然科学基金(31050002)
山西省自然科学基金(2010011050-1)
山西医科大学科技创新基金(01200904)
关键词
牙周炎
动脉粥样硬化
牙周干预
超敏C反应蛋白
Periodontitis
AtheroseIerosis
Periodontal intervention
High sensitivity C- reactive protein
