摘要
目的通过研究经羟基磷灰石(HA)涂层处理后多孔镍钛(NiTi)合金的溶血率及成骨细胞在其表面的附着、增殖及分化情况,评价其体外生物相容性。方法测定经HA涂层处理的圆盘状多孔NiTi合金(NiTi-HA组;直径10mm,厚2mm)的生物相容性,未经涂层处理的多孔NiTi合金(NiTi组)及致密纯钛(Ti组)试样设为对照组。采用分光光度法分析其溶血性能;将成骨细胞接种于试样表面,用扫描电镜(SEM)观察成骨细胞黏附形态,MTS法及碱性磷酸酶(ALP)试剂检测细胞附着、增殖情况及ALP活性,对数据进行重复测量方差分析。结果 NiTi-HA组、NiTi组及Ti组试样的溶血率分别为(0.30±0.11)%、(0.51±0.07)%及(0.27±0.06)%,均低于国家标准(YY/T0127.1)规定的5%。SEM观察显示,NiTi-HA组及NiTi组试样细胞黏附形态良好。NiTi-HA组试样表面细胞附着及增殖数量均高于NiTi组试样(P值分别为0.000与0.001)。NiTi-HA组及NiTi组试样表面细胞ALP活性无差异(P=1),但均高于Ti组试样(P值分别为0.001与0.0004)。结论 NiTi-HA组、NiTi组及Ti组试样均无溶血作用;NiTi-HA组较NiTi组更有利于成骨细胞附着和增殖,且ALP活性均高于纯钛。
Objective To investigate the biocompatibility of the HA coated porous NiTi alloys in vitro. Hemolysis rate, cell attachment, proliferation, and ALP activity of the human fetal osteoblastic 1.19 cell line （hFOB 1.19） cultured on the HA coated porous NiTi alloys were measured. Methods A series of biocompatibility tests of porous NiTi alloy disc-shaped samples （ 10 mm in diameter and 2 mm in thickness） coated with HA were performed, and the untreated porous NiTi alloy and pure titanium samples were used for a comparative study. The hemolysis of all samples was tested by spectrophotometry. Scanning electron microscope （SEM） was used to observe morphology of hFOB 1.19 incubating on the samples, and MTS method and ALP kit were used to test cell attachment, proliferation and ALP activity. Repeated measurements were carried out with analysis of variance on the data. Results Hemolysis rates of the untreated NiTi alloy, HA-coated porous NiTi alloy and pure titanium samples are （0.30 ± 0.11 ）%, （0.51 ±0.07）% and （0.27 ± 0.06）%, respectively, which are lower than 5% and acceptable according to the YY/T0127.1 standard. The SEM observation revealed that the hFOB 1.19 attached and spreadwell on the HA-coated porous NiTi alloy. The cell attachment and cell proliferation tests results showed that cells were better attached （P = 0.000） and proliferated （P = 0.001） on the HA-coated NiTi alloy samples than on the untreated NiTi alloy samples. ALP activity experimental results showed no significant difference between the HA-coated and untreated NiTi sample groups （P= 1）, but the samples in both groups exhibited higher ALP activity than the pure titanium samples （P= 0.001, P= 0.0004）, respectively. Conclusions The results indicated that the HA-coated NiTi alloy and untreated NiTi alloy samples showed no hemolysis effect; HA coating can enhance the attachment and proliferation of hFOB 1.19 on the porous NiTi alloy, and ALP activity of the HA-coated and untreated porous NiTi alloys was higher than that of pure Ti.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2012年第3期34-38,共5页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
国家自然科学基金(50871039)
广东省自然科学基金(10151064101000017)
