摘要
目的探讨不同类型淋巴瘤患者肿瘤坏死因子-β(TNF-β)在肿瘤组织与血浆中表达及其临床意义。方法收集97例不同类型淋巴瘤患者石蜡组织和血浆标本,应用免疫组化和ELISA法观察组织与血浆中TNF-β表达情况。结果 TNF-β在霍奇金淋巴瘤(HL)、弥漫大B细胞淋巴瘤(DLBCL)、浆细胞肿瘤(PCN)、小淋巴细胞淋巴瘤/慢性淋巴细胞白血病(SLL/CLL)、滤泡性淋巴瘤(FL)、周围T细胞淋巴瘤(PTL)表达较高,而在淋巴母细胞淋巴瘤(LBL)、结外NK/T细胞淋巴瘤(NK/TCL)表达较低,各种类型淋巴瘤组织TNF-β的表达情况比较,差异无统计学意义(P>0.05)。在HL、DLBCL、PCN、SLL/CLL、FL、PTL患者血浆中TNF-β水平高于健康对照组,差异有统计学意义(P<0.05)。组织中TNF-β表达在国际预后指标(IPI)评分的中、高危组与低危组比较,差异有统计学意义(P<0.05)。TNF-β在组织与血浆中的表达无相关性(rs=0.12,P>0.05)。结论 TNF-β在淋巴瘤组织与血浆中都有不同程度表达,提示TNF-β在淋巴瘤发生、发展中起到一定作用,但是TNF-β与淋巴瘤的关系比较复杂,不同的类型可能存在不同的机制。
Objective To investigate the expression of tumor necrosis factor β（TNF-β） in tissue and plasma of subtypes of lym- phoma and its significance. Methods Immunohistochemical technique and enzyme-linked immunosorbent assay（ELISA） were re- spectively used to assess the expression of TNF-β in 97 cases paraffin tissue and plasma samples of subtypes of lymphoma. Results Expressions of TNF-β were higher in HL, DLBCL, PCN, SLL/CLL, FL and PTL then in LBL, and NK/TCL. Expressions of TNF-β in tissue of subtypes of lymphoma had not significant difference（P〉0.05）. Expression of TNF-β in plasma of HL,DLBCL, PCN, SLL/CLL,FL and PTL were higher than control group,TNF-β in these lymphoma were significant difference（P〈0.05）. Ex- pressions of TNF-β in tissue had significant difference between low-risk group and middle or high risk group of international prog- nosis index（P〈0.05）. Expressions of TNF-β in tissue and plasma of subtypes of lymphoma had no correlation（rs= 0. 12, P〉 0.05）. Conclusion TNF-β has been found various degree expressions in tissue and plasma of lymphoma,TNF-β play a role in the development of lymphoma, but the expression of TNF-β in subtypes of lymphoma may exist different mechanisms.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第18期1795-1798,F0002,共5页
Chongqing medicine
基金
国家自然科学基金支持项目(30860096)
