摘要
目的：研究原发性肝细胞性肝癌中ｐ１６基因的状态。方法：采用多重ＰＣＲ分析法、ＳＳＣＰ分析法及以ＰＣＲ为基础的甲基化分析法分别对四川地区人群２５例原发性肝细胞性肝癌标本及其相应的癌旁组织中ｐ１６基因的缺失、突变及甲基化情况进行了检测。结果：２５例肿瘤标本中，发现３例ｐ１６基因缺失，其中２例纯合子缺失，１例半合子缺失，未发现１例突变，在未发现基因缺失的２２例肿瘤标本中发现６例有ｐ１６基因高甲基化存在。全部相应的癌旁组织中均未发现有ｐ１６基因缺失、突变或甲基化存在。结论：ｐ１６基因的异常改变在原发性肝癌的发生与发展中可能起重要作用，其改变以甲基化为主。
Objective: To investigate the status of p16 gene in primary hepatocellular carcinoma (HCC). Methods: A total of 25 HCC samples with correspondant tumor-adjacent specimens were screened for p16 gene deletions, mutations and hypermethylations, by using multiplex polymerase chain reaction (PCR), single strand conformational polymorphism(SSCP) and PCR based methylation analysis, respectively. Results: Among 25 HCC samples, 2 homozygous and 1 hemizygous deletions and 6 hypermethylations were detected and no mutations were idendtified. However, no deletions, mutations or hypermethylations were found in all the tumor-adjacent tissues. Conclusion: The abnormal alterations of p16 gene may play an important role in the carcen genesis of primary hepatocellular carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第1期45-47,共3页
Chinese Journal of Cancer
关键词
P16基因
甲基化
肝肿瘤
基因突变
Primary hepatocellular carcinoma
p16 gene
Deletion
Mutaion
Hypermethylation.
