摘要
目的:探讨慢性胃炎患者腻苔形成过程中舌苔代谢物质的变化。方法:利用人工与Z-BOX舌象数字化分析仪共同判读的方法诊断受试者的舌苔,其中慢性胃炎患者腻苔40例,非腻苔30例及正常人淡红舌薄白苔20例。采集舌苔样本,经前期处理后,采用高效液相色谱质谱联用技术检测各组患者舌苔中的代谢成分,得到代谢指纹图谱,并对其进行主成分分析、偏最小二乘法判别、正交偏最小二乘法判别分析,得到潜在的代谢标志物,并利用ChemSpider、HMDB(Human Metabolome Database)网站检索,对潜在标志物进行初步鉴定。结果:正交偏最小二乘法判别分析发现,慢性胃炎腻苔组、非腻苔组和正常组舌苔的代谢产物分布在不同区域内。进一步分析代谢物后发现,腻苔组和非腻苔组之间差异的化合物有3-酮基乳糖、2-脱氧-D-核糖、UDP-D-半乳糖、变视紫红(质)、抗坏血酸盐、吡啶甲酸、组氨酸;腻苔组和正常组之间差异的化合物有3-酮基乳糖、UDP-D-半乳糖、白细胞三烯A4、维生素D2等。结论:慢性胃炎腻苔与非腻苔患者及正常舌苔间代谢物质有明显差异,这些代谢物质主要参与能量代谢,以糖代谢为主。糖代谢的变化是腻苔形成的物质基础之一。
OBJECTIVE：To explore the changes in metabolites in the greasy tongue coating in patients with chronic gastritis. METHODS：Forty chronic gastritis patients presenting with greasy tongue coating,30 chronic gastritis patients presenting with non-greasy tongue coating,and 20 healthy control persons presenting with light red tongues and thin white coating were enrolled,and the tongue coating was detected by combining artificial diagnosis and the Z-BOX Tongue Digital Analyzer’s diagnosis.Samples of all the tongue coatings were collected before treatment.The metabolic fingerprinting of the tongue coating samples was obtained using ultra-performance liquid chromatography and mass spectrometry（UPLC-MS）,and the metabolic components in the tongue coating samples were detected.After this,principal component analysis,partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis（OPLS-DA）were used to identify potential metabolic markers.Finally,the components were identified using the Chemspider and HMDB searching. RESULTS：UPLC-MS results were analyzed by OPLS-DA and showed that the metabolites among the three groups were distributed in different regions.The different potential metabolic markers between the patients with or without greasy coating were 3-ketolactose,2-deoxy-D-ribose,UDP-D-galactose metarhodopsin, ascorbate,picolinate and histidine.The different potential metabolic markers between the greasy coating group and the normal group were 3-ketolactose,UDP-D-galactose,leukotriene A4 and vitamin D2. CONCLUSION：The metabolites of the greasy coating group,the non-greasy coating group and the normal group show significant differences in energy metabolism,mainly of glucose metabolism.This demonstrated that glucose metabolism may be one of the mechanisms leading to the formation of greasy coating.
出处
《中西医结合学报》
CAS
2012年第7期757-765,共9页
Journal of Chinese Integrative Medicine
基金
上海高校选拔培养优秀青年教师科研专项基金资助项目(No.szy08001)
上海中医药大学校级杏林学者基金资助项目(No.R09205)
上海市(第三期)重点学科中医诊断学资助项目(No.S30302)
关键词
腻苔
慢性胃炎
代谢组学
液相色谱质谱联用技术
临床对照试验
greasy tongue fur
chronic gastritis
metabolomics
liquid chromatography and mass spectrometry
controlled clinical trial
