摘要
目的观察4种烤瓷基底冠金属浸提液对人牙龈成纤维细胞分泌前列腺素E2和环加氧酶2的影响。方法制备镍铬合金、钴铬合金、纯钛、金钯合金金属浸提液，以无金属浸泡的达尔伯克改良伊格尔培养基培养液作为空白对照，培养人牙龈成纤维细胞，分别培养1、6、12、24h，收集上清液，酶联免疫吸附测定法测定前列腺素E2的表达水平；制作细胞爬片，用免疫荧光法观察各组环加氧酶2的表达。结果镍铬合金、钴铬合金组6、12、24h前列腺素E2表达水平（镍铬：45．568±0．926、60．538±0．988、73．754±0．507；钴铬：40．496±0．693、53．216±0．327、65．470±1．086）高于相应空白对照组（31．122±0．642、31．230±0．634、30．980±0．746），差异均有统计学意义（P〈0．05）；纯钛、金钯合金组前列腺素E：表达水平（钛：31．564±0．719、31．998±0．856、32．066±0．513；金钯：31．540±0．821、31．136±0．518、31．340±0．443）与相应空白对照组差异均无统计学意义（P〉0．05）。镍铬合金、钴铬合金组环加氧酶2的染色深而均匀；纯钛、金钯合金组与空白对照组染色浅且不均匀。结论镍铬合金、钴铬合金使人牙龈成纤维细胞分泌前列腺素E2和环加氧酶2增加，而纯钛、金钯合金不影响前列腺素E2和环加氧酶2的分泌。
Objective To investigate the effect of alloy leaching liquor of four different types of base metal alloy on the expression of prostaglandin E2 （ PGE2 ） and cyclo-oxygenase-2 （COX-2） by human gingival fibroblast（HGF） in vitro. Methods Ni-Cr, Co-Cr, pure Ti and Au ceramic alloys were incubated in Dulbecco＇s modified Eagle＇s medium （DMEM） to prepare alloy leaching liquor, and then added in HGF medium. DMEM was prepared as negative control Aliquots were taken from exposed media after 1, 6, 12, 24 h. Assays for PGE2 were carried out by enzyme-linked immunosorbent assay （ELISA）. Results In 6, 12, 24 h, the expression of PGE2 in Ni-Cr and Co-Cr alloy groups （ Ni-Cr： 45. 568 ± 0.926, 60. 538-±0.988, 73. 754 ±0.507; Co-Cr： 40. 496 ±0.693, 53. 216 ±0.327, 65. 470 ± 1.086） were significantly higher than those in other experimental groups （ Ti： 31. 564 ± 0. 719, 31. 998 ± 0. 856, 32.066±0.513; Au alloy： 31.540±0.821, 31.136±0.518, 31.340 ±0.443） and control group （31. 122 ±0. 642, 31. 230 ±0. 634, 30. 980 ±0. 746） （P 〈0. 05）. No significant difference were found in the expression of PGE2 among pure Ti, Au alloy groups and the control group （P 〉 0.05 ） . Immunofluorescence showed dark and uniform COX-2 stain in Ni-Cr and Co-Cr alloy groups, while in pure Ti group, Au alloy group, and negative control group shallow and uneven distribution of COX-2 stain were observed. Conclusions Our findings suggested that pure Ti and Au alloy did not cause elevated PGE2 and COX-2 release from HGF. However, Ni-Cr and Co-Cr alloy caused increase in PGE2 and COX-2 levels.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2012年第7期431-434,共4页
Chinese Journal of Stomatology
