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cDNA-RDA方法筛选与分析VBNC状态沙门菌差异基因的研究 被引量：2

The study for screening and analyzing of the differential genes of Salmonella in the VBNC state using cDNA-RDA
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摘要 为了研究VBNC状态沙门菌特异性基因,探讨细菌VBNC状态发生机制,试验应用cDNA代表性差异分析技术(cDNA-RDA)对VBNC状态沙门菌特异性基因进行筛选及分析。结果表明:经过3轮差减杂交后,获得8条差异基因片段;分析差异基因功能,推测其中5条差异基因可能与沙门菌VBNC状态发生机制以及合成蛋白相关。 To study the Salmonella-specific genes in the VBNC state,and to explore the bacterial VBNC mechanisms,The method of cDNA-RDA was used to screen and analyze the Salmonella-specific gene in the VBNC state.The results showed that after three rounds subtractive hybridization,eight differential gene fragments were obtained.It was presumed that among them,there were five differential genes that may be related to the occurrence mechanism of VBNC and protein synthesis using the functional analysis of differential genes.
作者 胡烨
机构地区 天津农学院
出处 《黑龙江畜牧兽医》 CAS 北大核心 2012年第7期19-22,共4页 Heilongjiang Animal Science And veterinary Medicine
关键词 沙门菌 活的非可培养状态 cDNA代表性差异分析 差异基因 Salmonella viable but not-culturable state cDNA representational difference analysis differential genes
分类号 S852.612 [农业科学—基础兽医学]
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参考文献13

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共引文献12

同被引文献48

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黑龙江畜牧兽医
2012年 第7期

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