摘要
目的分析河南省部分地区病毒性脑炎的病原种类及流行特点。方法对2008年新乡、开封、洛阳监测的病毒性脑炎病例进行流行病学调查,采集血液和脑脊液标本,采用ELISA方法检测流行性乙型脑炎(乙脑)IgM,对其中乙脑IgM阴性病例脑脊液标本进行细胞培养分离,采用逆转录聚合酶链反应(RT-PCR)检测其他病毒。结果从215例病毒性脑炎病例中检出乙脑IgM抗体阳性47例,阳性率为21.86%;从乙脑IgM阴性的168例脑脊液中分离到肠道病毒24株,阳性率为14.29%;24株肠道病毒中,埃可病毒30(ECHO30)12株,埃可病毒6(ECHO6)7株,埃可病毒25(ECHO25)4株,柯萨奇病毒B5(CoxB5)1株;47例乙脑和168例肠道病毒等病毒性脑炎发病高峰为7～9月,分别占93.62%和70.24%;新乡、开封的乙脑和肠道病毒等病毒性脑炎以0～14岁儿童为主,分别为91.91%、92.31%和97.92%、98.41%;洛阳的肠道病毒等病毒性脑炎0～14岁占92.99%,乙脑≥15岁发病明显上升,占56.52%,差异有统计学意义(χ2=21.13,P<0.05);洛阳乙脑病例以农民为主,新乡、开封乙脑病例及3地肠道病毒等病毒性脑炎病例均以散居、托幼儿童和学生为主。结论病毒性脑炎病原体以乙脑病毒和肠道病毒为主;乙脑与肠道病毒等病毒性脑炎高发季节一致;不同地区2类脑炎发病年龄、职业有差别。
Objective To analyze pathogen species and prevalent characteristics of the viral encephalitis in some areas of Henan province.Methods Surveillance data on all viral encephalitis(VE)cases diagnosed in 2008 were collected from Xinxiang,Kaifeng,and Luoyang.The blood and cerebrospinal fluid(CSF) samples were collected from the cases and IgM antibody against Japanese encephalitis(JE) was detected with enzyme-linked immunosorbent assay(ELISA).The antibody of other virus was detected with reverse transciption-PCR(RT-PCR).Cell culture separation was conducted for IgM antibody negative specimens.Results A total of 215 viral encephalitis cases were reported and 47(21.86%) cases were positive for IgM antibody against JE.A total of 24(14.29%) intestinal virus strains were isolated from the cerebrospinal fluid of 168 IgM antibody negative cases.Among the the virus,there were twelve of ECHO30,seven of ECHO6,four of ECHO25,and one of CoxB5.The 47 JE and 168 intestinal virus and other viral encephalitis cases occurred mainly in July to September,accounting for 93.62% and 70.24% of the cases.The 0-14 years old group of JE and intestinal virus and other virus encephalitis cases occurred mainly in Xinxiang(91.91% and 92.31%) and Kaifeng(97.92% and 98.41%).The 0-14 years old cases of intestinal virus and other viral encephalitis cases accounted for 92.99% of all cases in Luoyang city and≥15 years old cases of JE significantly increased(56.52%).The incidences of JE,intestinal virus,and other viral encephalitis were significantly different between 0-14 years old group and≥15 years old group(χ2= 21.13,P<0.05).The JE cases were mainly farmers in Luoyang and the JE cases of intestinal virus and other viral encephalitis in Xinxiang and Kaifeng were mostly scattered children,children in and kindergarten,or students.Conclusion Japanese encephalitis virus and intestinal virus are main pathogens of viral encephalitis in Xinxiang,Kaifeng,and Luoyang.The peak season of the intestinal virus and other viral encephalitis was the same as that of JE.The age and occupation distribution of the cases are different in different areas.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第7期967-970,共4页
Chinese Journal of Public Health
基金
河南省医学科技攻关项目(200702016)
关键词
病毒性脑炎
乙脑病毒
肠道病毒
viral encephalitis
Japanese encephalitis virus
intestinal virus
