摘要
目的:建立穿山龙总皂苷的高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。方法:测定9批不同来源的穿山龙样品。色谱柱为Kromasil C8(250mm×4.6mm,5μm),流动相为水-乙腈(梯度洗脱),流速为1.0mL·min-1,检测波长为203nm,柱温为30℃。结果:共标示出穿山龙皂苷类化合物17个特征指纹峰;不同来源的穿山龙总皂苷色谱峰峰面积比值有一定的差异。结论:HPLC指纹图谱分析法能简便、快速地区分不同来源的穿山龙,为全面控制穿山龙药材的质量提供依据。
OBJECTIVE： To establish the HPLC fingerprints for total saponins from Dioscorea nipponica, and to provide refer- ence for quality evaluation of it. METHODS： 9 batches of D. nipponica collected from different regions were determined. The sepa- ration was performed on Kromasil C8（250mm×4.6mm,5μm） column using water-acetonitrile （gradient elution） as mobile phase at flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 203 mn and the column temperature was 30 ℃. RESULTS： 17 peaks were identified as the characteristic fingerprints of saponins from D. nipponica and the ratio of peak area of different sam- pies were different. CONCLUSION： The method is suitable to differentiate D. nipponica from different regions conveniently, and can provide reference for quality control of D. nipponica completely.
出处
《中国药房》
CAS
CSCD
2012年第27期2555-2557,共3页
China Pharmacy
