摘要
目的:从肾气丸"阴中求阳"配伍对肾阳虚模型大鼠HPG轴的影响,初步探讨其"阴中求阳"的配伍机理。方法:将SD雄性大鼠随机分为正常对照组、模型组、补阳组、补阴组、阴阳双补组,观察大鼠一般活动。大腿内侧肌肉丰厚处注射氢化可的松12天制造肾阳虚模型。成模后各组灌胃治疗20天,第21天上午8时取血,采用酶联免疫法(ELISA)检测血清PRL、FSH、LH、E2、T水平。取睾丸、附睾、精囊、前列腺称重,测睾丸、附睾、精囊、前列腺重量指数。结果:阴阳双补组能增加肾阳虚模型大鼠体重,升高睾丸、附睾、精囊、前列腺重量指数,改变血清PRL、FSH、LH、E2、T水平,其作用明显优于补阳组或补阴组。补阳组与补阴组比较,在增加模型大鼠体重方面,补阳组效果优于补阴组;在升高睾丸、附睾、精囊、前列腺重量指数方面,补阴组效果略微优于补阳组,但二者之间差异不明显。各治疗组中,补阳组效果仅在影响PRL、LH水平方面有统计学意义。结论:针对肾阳虚证,单纯补阳、补阴效果不明显,阴阳双补可提高疗效,初步阐释了肾气丸"阴中求阳"的配伍理论。
Objective：To study the compatibility mechanism of ＂reinforcing Yang from Yin＂ from the effect of Compdttibility of Shenqi Pill ＂reinforcing Yang from Yin＂ on HPG of Kidney-Yang deficiency model rats. Methods ： SD male rats were randomly divided into five groups ： normal, model, warming Yang, nourishing Yin, warming Yang and nourishing Yin, and then obserated the activity.The rat model with Kidney-Yang deficiency was induced by injecting hydrocomsone at the inside of the leg muscles for 12 days. After the models were established, the rats were treated with different combinative drugs with Shenqi Pill for 20 days. All the rats were killed at 21st day ,the level of PRL, FSH, LH, E2, T were detected by ELISA ,and the weight of Testis, Epididymis, Prostate and Seminal vesicle were weighed to detect the index of the weight of Testis, Epididymis, Prostate and Seminal vesicle. Results ： In warming Yang and nourishing Yin group, the weight of rat body and the index of the weight of testis, epididymis, prostate and seminal vesicle increased much more distinctly, and the level of PRL, FSH, LH, E2, T changged, compared with thai of warming Yang group or nourishing Yin group,the effect was better. At the same time, in warming Yang group, the weight of rat body increased more than that of nourishing Yin group, and between the two groups, it was no significant difference on the effect of the index of the weight of Testis, Epididymis, Prostate and Seminal vesicle increased. In all the groups, the effect of wanning Yang group changged the level of PRL, LH. Conclusion ： For Kidney-Yang deficiency,it is worse to warm Kidney-Yang or nourishe Kidney-Yin only, warming Kidney-Yang and nourishing Kidney-Yin could improve the Kidney-Yang deficiency model rats. At an early stage, the experiment explains the compatibility mechanism of ＂reinforcing Yangfrom Yin＂ .
出处
《辽宁中医药大学学报》
CAS
2012年第7期263-265,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
肾气丸
阴中求阳
肾阳虚
HPG
Shenqi Pill
Reinforcing Yang from Yin
Kidney-Yang deficiency
HPG
