摘要
目的探讨Twist和HIF-1α在鼻咽癌组织中表达及其相关性。方法应用RT-PCR及SABC免疫组化法检测50例鼻咽癌组织Twist、HIF-1α的mRNA及蛋白表达情况。结果鼻咽癌组织中不同患者年龄、肿瘤大小分组间Twist及HIF-1α的mRNA和蛋白表达差异无统计学意义;不同淋巴结转移、临床分期和病理分级分组间Twist及HIF-1α的mRNA和蛋白表达差异具有统计学意义。鼻咽癌组织中Twist的mRNA及蛋白表达间、HIF-1α的mRNA及蛋白表达间、Tw ist和HIF-1α的蛋白表达间、Tw ist和HIF-1α的mRNA表达间均存在正相关关系。结论鼻咽癌组织中Twist、HIF-1α均高表达,且两者间具有相关性。HIF-1α蛋白可能通过上调Twist表达成为其促进鼻咽癌浸润、转移的途径之一。
Objective To explore the relationship of Twist and hypoxia inducible factor-1 alpha （HIF-1α） in nasopharyngeal carcinoma（NPC） . Methods Expressions of Twist and HIF-1α were detected in 50 cases of NPC by PCR and immunohistochemistry. Results The positive Twist and HIF-lct expressions were 86. 0% and 81. 0% in NPC. There was a significant correlation between them （ P 〈 0. 01 ）. Conclusion Over-expressions of Twist and HIF-1α protein were found in NPC. To stimulate Twist may be one of the pathways of HIF-1α protein to contribute to the invasion and metastasis in human NPC.
出处
《山东大学耳鼻喉眼学报》
CAS
2012年第4期30-33,共4页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
深圳市科技创新委资助课题(201002052)
