摘要
目的近年来抗生素及免疫抑制药物滥用、器官移植等使得真菌感染的发生率逐渐增高。自然环境恶化也成为危险因素,其中包括紫外线照射。文中构建模拟日光照射导致皮肤光损伤动物模型及光损伤后须癣毛癣菌感染动物模型,研究模拟日光照射剂量与须癣毛癣菌感染情况的关系。方法利用SUV-1000日光紫外线模拟器照射豚鼠背部皮肤,测出其最小红斑量(minimal erythema dose,MED)。将实验豚鼠分为5组,第1、2、3、4组分别以0MED、0.5MED、1MED、4MED连续3 d照射其背部皮肤。照射完成后次日,将预先制备的须癣毛癣菌悬液均匀涂抹于照射部位皮肤,第5组为空白对照组,脱毛后不进行照射,背部涂抹等渗盐水以作对照。通过皮肤真菌镜检、培养和组织病理方法验证感染结果,给予感染后皮损评分,观察时间为4周。结果豚鼠背部皮肤模拟日光平均MED总剂量为1552 mJ/cm2,其中紫外线B(ultraviolet B,UVB)122mJ/cm2,相应紫外线B(ultraviolet A,UVA)1430mJ/cm2。模拟日光照射后第4组皮肤红斑反应最明显,第3组红斑程度较轻,第1、2组未见红斑。第1、2、3、4组豚鼠照射后真菌感染均成功,阳性率100%。第4组与1、2组皮损评分之间差异有统计学意义(P<0.05),与第3组差异无统计学意义(P>0.05);1、2、3组之间比较差异均无统计学意义(P>0.05)。结论以≥MED的模拟日光照射后,豚鼠皮肤可发生日晒红斑反应;模拟日光照射剂量对真菌感染的发生率无影响,但对真菌感染后皮肤损害的严重程度及皮损自愈有显著影响。为模拟紫外线照射对皮肤真菌感染的形成作用机制研究成功建模。
Objective With the abuse of antibiotics and e agents, organ transplantation and aggravation of environment, the incidence of fungal infection has been on the rise in the past few years. This experiment was to construct an animal model of light damage caused by solar-simulated ultraviolet （SSUV） and an animal model of Trichophyton mentagrophyte infection （TMI） after light damage, and to study the relationship between the dose of SSUV and TMI. Methods We determined the average minimal erythema dose （MED） for guinea pigs by exposing their back skin to SUV-IO00 SSUV. We established a model of TMI by exposing four groups of guinea pigs to 0MED, 0.5MED, 1MED and 4MED of SSUV respectively for 3 consecutive days, followed by application of Tricho- phyton mentagrophyte suspension on the irradiated area the fourth day, while the fifth group as the blank control remained unexposed and received physiological saline instead. We evaluated the resuhs of model construction by directmicroscopy, culturing and histopathologic method, and recorded the scores on post-infection skin lesion for 4 weeks. Results The average MED of SSUV in the back skin of the guinea pigs was 1552 mJ/cm^2 of the total dose （ UVB ： 122 mJ/cm^2 ; UVA ： 1430 mJ/cm^2）. UVR-evoked skin erythema was the most obvious in the 4MED group, and then came the 1MED group, but none was found in the 0MED and 0.5MED groups. TMI was induced in 100% of the four groups of guinea pigs after SSUV irradiation. The 4MED group showed statistically significant difference in the skin lesion score from the 0MED and 0.5MED groups （ P 〈 0.05 ）, but not from the 1MED group （ P 〉 0.05 ）, nor were any significant differences observed among the 0MED, 0.5 MED and 1MED groups （ P 〉 0.05 ）. Conclusion Skin erythema could be evoked by exposure to SSUV at ≥ 1MED. The dose of SSUV irradiation has a significant impact on the severity and self-healing of skin lesion after fungal infection, but not on the incidence of fungal infection. An animal model was suc- cessfully constructed for the study of the mechanism of SSUV inducing fungal infection.
出处
《医学研究生学报》
CAS
北大核心
2012年第6期577-581,共5页
Journal of Medical Postgraduates
基金
国家自然科学基金(81071331)
关键词
模拟日光照射
最小红斑量
真菌感染
须癣毛癣菌
Solar-simulated radiation
Minimal erythema dose
Fungal infection
Trichophyton mentagrophyte
