摘要
目的通过检测慢性乙型肝炎病毒(HBV)感染者外周血中白细胞介素-35(IL-35)水平,探讨IL-35在HBV持续感染致病机制中的作用。方法用酶联免疫吸附试验(ELISA)法检测27例慢性乙型重型肝炎(CSHB)患者,69例慢性乙型肝炎(CHB)患者,24例乙型肝炎肝硬化(LC)患者,29例慢性乙型肝炎病毒携带者(ASC)和26例健康者作为正常对照组(NC)的外周血中IL-35水平,并对不同组别患者之间的IL-35水平进行比较;对IL-35水平与HBV DNA载量、肝功能指标ALT、AST、ALB和TBil进行了相关性分析。结果 HBV感染者中IL-35水平高于NC组,其中CSHB组患者IL-35水平(19 408±23 272.6 pg/ml),显著高于CHB组(4478.1±5838.9 pg/ml,P<0.05)、LC组(3920.9±6520.2 pg/ml,P<0.05)、ASC组(224.7±123.3 pg/ml,P<0.05)及NC组(37.0±48.6pg/ml,P<0.05);CHB组IL-35水平显著高于ASC和NC组(P<0.05),与LC组无显著性差异;ASC组显著高于NC组(P<0.05);IL-35水平与ALT、AST、TBil和PT水平呈正相关(P<0.05),与ALB水平呈负相关(P<0.05);与HBV DNA载量无显著线性相关,HBeAg阳性与阴性者患者血清中IL-35水平比较差异无统计学意义。结论 HBV感染者外周血中IL-35水平增高,提示IL-35在HBV持续感染的免疫致病机制中起着一定作用,为临床中通过调控IL-35水平来治疗HBV感染提供了新的思路。
Objective To investigate the value of interleukin-35 （IL-35） in the pathogenesis of hepatitis B virus （HBV） continuous infection by detecting the expression levels of IL-35 in patients with HBV infection. Methods The levels of IL-35 in the peripheral blood of 27 cases with chronic severe hepatitis B （CSHB）, 69 cases with chronic hepatitis B （CHB）, 24 cases with liver cirrhosis （LC）, 29 cases with asymptomatic carriers （ASC） and 26 normal controls （NC） were detected by enenzyme-linked immunosorbent assay （ELISA）, respectively. The levels of IL-35 in different groups were compared. The correlation between IL-35 and liver function such as ALT, AST, TBil, ALB and HBV DNA was studied. Results The levels of IL-35 in patients with HBV infection were significantly higher than those in NC group. The levels of IL-35 in CSHB were significantly higer than those in CHB, LC, ASC and NC groups （ P 〈 0.05 ）. The levels of IL-35 in CHB were significantly higher than those in ASC and NC groups （ P 〈 0.05） , but there was no significant difference between CHB and LC groups. And levels of IL-35 in ASC were significantly higher than those in NC group（ P 〈 0.05 ）. The levels of IL-35 were positively correlated with ALT, TBil and PT, but negatively correlated with ALB and not significantly correlated with AST and HBV DNA. Thre were no significant difference in levels of IL-35 between patients with HBeAg positve and HBeAg negative. Conclusions The higher levels of IL-35 in peripheral blood of patients with HBV infection indicates that IL-35 might play an important role in the pathogenesis of HBV continuous infection and could provide a new idea for rescuing HBV infection by regulating IL-35.
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2012年第3期33-36,共4页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
2011年江苏省"科教兴卫"医学重点人才培养基金(No.RC2011117)
2011年江苏省第四期"333高层次人才培养工程"第三层次培养对象基金
