摘要
目的:研究罗格列酮对多柔比星所致肾脏氧化应激损伤大鼠肾脏的保护作用并探讨其作用机制。方法:采用MTT法测定不同浓度的罗格列酮对多柔比星作用下细胞的增殖活性的影响。50只雄性SD大鼠随机分为空白对照组、多柔比星对照组和罗格列酮治疗组,大鼠尾静脉一次性注射多柔比星6.5 mg.kg-1制备多柔比星肾病模型。1周后,空白对照组每天蒸馏水灌胃,治疗组每天分别灌胃给予20、10和5mg.kg-1罗格列酮,持续8周。8周后检测大鼠血清尿素氮(BUN)和肌酐(Scr)水平及肾组织各氧化指标,同时取肾组织标本观察病理学改变。结果:用罗格列酮孵育能够明显提高DOX作用下HEK29细胞存活率;与模型对照组相比,罗格列酮20 mg.kg-1组血清肌酐、尿素氮明显下降(P<0.05),肾组织中NO含量、NOS和SOD活性明显上升、MDA含量明显下降(P<0.05),肾组织病理损伤减轻。结论:罗格列酮能降低多柔比星所致大鼠肾脏氧化应激损伤,具有肾保护作用。
Objective: To observe the protective effects of rosiglitazone in rats with doxorubicin-induced nephropathy and its anti-oxidative mechanism.Method: The effects of rosiglitazone and doxorubicin on the in vitro growth of HEK 293 cells were observed by MTT assay.Then fifty male SD rats were randomly divided into the control group,model group and rosiglitazone-treated group.The models of doxorubicin-induced nephropathy were established by tail vein injection of 6.5 mg·kg-1 doxorubicin.After one week,the model group was given distilled water and the rosiglitazone-treated group was fed with rosiglitazone(20,10 and 5 mg·kg-1).After consecutive 8 weeks,serum urea nitrogen and creatinine were assessed,and NO concentration,MDA,GSH-Px and SOD contents in the renal cortex were determined and histopathological changes in renal tissues were detected.Result: Compared with the model group,serum urea nitrogen,creatinine concentration and renal concentration of MDA in the rosiglitazone 20 mg·kg-1 group decreased significantly(P<0.05),while the activity of SOD and GSH-Px increased significantly(P<0.05).Histopathology of mice in the model group showed renal tubular injury,basophilic change,atrophy,cystic expansion,and mild interstitial inflammatory infiltration while it only showed interstitial inflammatory infiltration in the rosiglitazone 20 mg·kg-1 group.Conclusion: Rosiglitazone exhibits protective effects in rats with doxorubicin-induced nephropathy.
出处
《中国药师》
CAS
2012年第6期756-759,共4页
China Pharmacist
关键词
罗格列酮
多柔比星
肾病
保护作用
Rosiglitazone
Doxorubiein
Nephropathy
Protective effects
