摘要
目的:建立高效毛细管电泳法分离3种碱性药物对映体。方法:采用未涂层熔融石英毛细管柱,30 mmol·L-1NaH2PO4缓冲溶液中加入羧甲基-β-糊精(CM-β-CD)作为背景电解质,可使3种碱性药物的对映体得到较好的分离。结果:在最佳条件下,吲达帕胺、盐酸异丙嗪和盐酸普萘洛尔的分离度(R)分别为4.79,2.45和6.90。结论:CM-β-CD对吲达帕胺、盐酸异丙嗪和盐酸普萘洛尔有很高的对映体选择性。该方法重现性好,简便、快捷。
Objective： To establish a high performance capillary electrophoresis method for the chiral separation of indapamide, promethazine hydrochloride and propranolol hydrochloride. Method ： The separations of the enantiomers were performed in an untreated fused-silica capillary tube with earboxymethyl-β-cyclodextrin （CM-β-CD） in 30 mmol · L-1 NaH2PO4 buffer as the chiral selector and a high voltage of 20 kV. Result ： Under the optimal conditions, the resolution of indapamide, promethazine hydrochloride and prop- ranolol hydrochloride was 4.79, 2.45 and 6.90, respectively. Conclusion： CM-β-CD is highly enantioselective for indapamide, promethazine hydrochloride and propranolol hydrochloride. The method is simple and fast with an improved reproducibility.
出处
《中国药师》
CAS
2012年第6期772-774,共3页
China Pharmacist
关键词
毛细管电泳
吲达帕胺
盐酸异丙嗪
盐酸普萘洛尔
手性分离
Capillary eletrophoresis
Indapamide
Promethazine hydrochloride
Propranolol hydrochloride
Chiral separation
