摘要
目的:评价不同采收期以及不同干燥方法对栀子苷的影响。方法:应用高效液相色谱法测定,色谱柱为Agilent Zor-bax XDB-C18色谱柱(150 mm×4.6 mm,5μm);流动相为乙腈-水(15∶85),流速1.0 ml·min-1;柱温:30℃;检测波长238 nm。结果:栀子苷的线性范围为0.31～155.76μg·ml-1(r=0.999 9),加样回收率为100.9%(RSD=1.2%);定量分析了6个采收时间、12种干燥方法的栀子中栀子苷含量。结论:不同采收时期、不同干燥方法对栀子中栀子苷的含量有一定的影响。
Objective： To study the effects of harvest time and drying methods on the geniposide content in Gardeniae Fructus. Method： An HPLC was applied with the following chromatographic conditions ： the chromatographic column was an Agilent Zorbax SB- C18 （150 mm×4.6 mm,5 μm） at 30℃, acetonitrile-water（ 15：85 ）as the mobile phrase, the flow rate was 1.0 ml · min-1, and the detection wavelength was 270 nm. Result： Linear relationship was within 0. 31-155. 76 μg · ml- 1 （r = 0. 999 9）, the average recovery was 100.9% （RSD = 1. 2% ）. The effects of 6 different harvest time and 12 drying methods on the geniposide content were quantitative- ly analyzed. Conclusion： The geniposide content can be influenced by different harvest time and drying methods significantly.
出处
《中国药师》
CAS
2012年第6期811-813,共3页
China Pharmacist
关键词
栀子
栀子苷
采收时期
干燥方法
Gardeniae Fructus
Geniposide
Harvest time
Drying methods
