摘要
目的:建立液相-质谱串联法测定人血浆中的沙丁胺醇含量。方法:血浆离心后过玻璃纤维滤膜,经酶解加入氘代沙丁胺醇内标溶液,用C18小柱净化后以3%的氨水甲醇溶液对Oasis MCX小柱进行洗脱,吹干,以0.1%甲酸水溶液-甲醇溶液(95∶5)溶解残余物,用液相质谱串联法测定,以0.1%甲酸乙腈溶液和0.1%甲酸溶液为流动相梯度洗脱。结果:沙丁胺醇在0.25～10.00μg.kg-1线性关系良好,检出限0.1μg.kg-1。结论:本法灵敏准确、重现性与特异性强、干扰少,可用于人体内沙丁胺醇药动学与生物利用度研究。
Objective ： To study the determination of salbutamol in human plasma by LC/MS/MS. Method： The deuterated salbu- tamol internal standard was added to the enzymatic hydrolyzed plasma samples after filtrated by glass fiber, then purified by Cls cartrid- ges and eluted by a solvent composed of 3% ammonia methanol through Oasis MCX cartridges. The eluent was dried and the remains were dissolved with 0.1% formic acid solution-95% methanol followed by LC/MS/MS determination with gradient elution by the mobile phase composed by 0.1% acetonitrile formate solution and 0.1% formic acid solution. Result： Salbutamol in human plasma samples was in good linear within the concentrations range of 0. 25-10. 00μg · kg^-1 with the detection limit of 0.1 μg · kg^-1. Conclusion： The method is sensitive, accurate, repeatable and specific with little interference, and suitable for the inspection of pharnmcokinetics and bioavailability of salbutamol in vivo.
出处
《中国药师》
CAS
2012年第6期837-840,共4页
China Pharmacist
