摘要
本试验选取对黑痣病菌抗性不同的马铃薯材料,研究黑痣病菌毒素对马铃薯幼苗体内丙二醛(MDA)含量、细胞膜透性及苯丙氨酸解氨酶(PAL)活性的变化及其与抗病性的关系。结果显示,经毒素处理后,马铃薯幼苗体内MDA含量大幅增加,96 h以前感病品种大西洋的累积量大于抗病品种底西芮的,96 h以后抗病品种的累积量大于感病品种的;马铃薯幼苗细胞膜透性增加,感病品种增加的较早且幅度较大;黑痣病菌毒素可诱导马铃薯幼苗体内PAL活性升高,抗病品种升高较早且幅度较大,抗病品种和感病品种的PAL活性分别在12 h和36 h达到最大,增幅分别为48.8%和38.7%,24 h以后,二者的增幅没有差异。
The relationship between the malondialdehyde（MI）A） content, cell membrane permeability, phcnylalanine ammonia lyase （ PAL ） activity and the resistance to Rhizoctonia solani in potato inoculated with it＇s toxin was studied with potato seedling of having different resistance. The results showed MDA content, cell membrane permeability and PAL activity were all increased after inocula- ting. 0 -96h, MDA content of susceptible cultivar Atlantic was greater than the resistance cuhivar Desirec~. 96 ~ 168h, MDA content of the resistance cultival： was greater than the susceptible cultivar. The cell membrane permeability of susceptible varieties increased earlier and more. PAL activity of resistant varieties increased earlier and more. Both reached maximum at 12h and 36h, rate of in- crease was 48.8% and 38.7%. 24h After inoculating, PAL activity has not significant different between resistant and susceptible vari- etie.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2012年第2期16-20,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
"十二五"国家科技支撑计划课题"马铃薯综合育种技术和多抗优质新品种选育"(2012BAD02B05)
内蒙古科技创新引导奖励资金项目(2012)
呼和浩特市农业综合开发科技创新项目(2011-计-2)
内蒙古自然科学基金(2010MS0305)
