摘要
目的观察补镁对2型糖尿病大鼠抗氧化能力和糖脂代谢的影响。方法高脂饲料喂养加链脲佐菌素注射方法制备2型糖尿病大鼠模型,然后氧化镁高、中、低剂量组在高脂饲料中分别加入氧化镁2 000、1 000、200mg/kg(以镁离子计)喂饲,糖尿病组只喂饲高脂饲料,正常对照组喂饲普通饲料。动物自由进食,连续4周,采用葡萄糖氧化酶法测定大鼠空腹尾尖血的血糖含量,腹主动脉取血测定空腹三酰甘油(TG)、血镁、胰岛素、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果与糖尿病组比较,氧化镁高剂量组SOD和T-AOC水平显著升高,而MDA、空腹血糖和TG水平明显降低,差异均有显著性(F=9.96～41.05,t=2.55～29.27,P<0.05)。结论补充镁可以提高2型糖尿病大鼠抗氧化能力,改善糖脂代谢。
Objective To investigate the effect of magnesium intake on anti-oxidative activity and glucolipid metabolisrrl in type-2 diabetic rats. Methods A model of type-2 diabetes was created in rats by feeding with high fat forage plus injection of streptozotocin. The rats in high-fat-diet groups were given magnesium oxide at a dose of 2 000, 1 000 and 200 mg/kg （based on magnesium ion） to rats in high-, median- and low-magnesium-oxide group. For those in model group, high fat diet only was given, those in the control group, fed with ordinary forage only. All the rats took food unconventionally for four weeks. Fasting blood glucose in tail ending was detected by glucose oxidase method. The blood taken from abdominal aorta was used to measure fasting triacylglycerol （TG）, the levels of magnesium, insulin, total antioxidative capability （T-AOC）, superoxide dismutase （SOD） and malondialdehyde （MDA）. Results Compared with the model group, SOD and T AOC in high-magnesium-oxide group obvious- ly increased, and MDA, blood glucose and TG decreased （F=9.96-41.05,t=2.55 29.27,P〈0.05）. Conclusion Supple- mentary of magnesium may increase anti-oxidative activityin rats with type-2 diabetes, and improve glucolipid metabolism.
出处
《青岛大学医学院学报》
CAS
2012年第4期295-297,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
镁
糖尿病
2型
超氧化物歧化酶
丙二醛
magnesium, diabetes mellitus, type 2
superoxide dismutase
malondialdehyde
