摘要
目的探讨Toll样受体4(TLR4)、P-糖蛋白(P-gp)在卵巢上皮癌组织中的表达及其临床意义。方法采用免疫组化SP法,检测50例卵巢上皮癌(化疗敏感组25例,化疗耐药组25例)组织和10例正常卵巢组织中TLR4、P-gp的表达。结果卵巢上皮癌组织中TLR4和P-gp的阳性表达率分别为62%和46%,明显高于正常卵巢组织的20%和10%(χ2=5.939、4.500,P<0.05)。卵巢上皮癌化疗耐药组TLR4和P-gp阳性表达率明显高于化疗敏感组(χ2=6.876、6.522,P<0.05)。卵巢上皮癌组织TLR4的表达与病理分级、临床分期和淋巴结转移有相关性(χ2=6.848、6.376、5.433,P<0.05),P-gp表达与病理分级、淋巴结转移具有相关性(χ2=9.071、6.402,P<0.05);TLR4、P-gp蛋白表达具有正相关性(r=0.392,P<0.05)。结论 TLR4和P-gp的高表达及其相关性与卵巢上皮癌的化疗耐药及预后关系密切。
Objective To study the expression and its clinical significance of TLR4 and P-gp in epithelial ovarian cancer （EOC） tissues. Methods Fifty cases of EOC were evenly divided into two groups as chemotherapy-sensittive group and chmo- therapy-resistant group, and 10 normal ovarian tissues were collected to serve as the control. The expression of TLR4 and P-gp was detected in the three groups by using immunochemistry SP method. Results The positive rates of TLR4 and P-gp in 50 EOC tissues were much higher than that in normal tissue,62% and 46% vs 20% and 10%, respectively （χ^2 =5. 939,4. 500;P~ 0.05）. The expressions of TLR4 and P-pg in chemotherapy-resistant group were much higher than that in the sensitivity group （χ^2 =6. 876,6. 522;P〈0.05）. The expression of TLR4 was correlated with pathology grading, clinical staging, and lymph node metastasis （χ^2 =6. 848,6. 376,5. 433 ;P〈0.05）, and that of P-gp was correlated with differentiation and lymph node metastasis （χ^2 =9. 071,6. 402 ;P〈0.05）. There was a positive correlation between the expressions of TLR4 and P-gp （r=0. 392 ,P〈0. 01）. Conclusion The high expression of TLR4 and P-gp and its correlation are closely associated with chemotherapy resistance and prognosis of epithelial ovarian cancer.
出处
《齐鲁医学杂志》
2012年第4期295-298,共4页
Medical Journal of Qilu
关键词
卵巢肿瘤
P糖蛋白
多药耐药相关蛋白质类
免疫组织化学
ovarian neoplasms
P-glycoprotein
multidrug resistance-associated proteins
immunohistochemistry
