摘要
目的 :探讨山莨菪碱联用地塞米松治疗多器官功能障碍综合征 ( MODS)的机制。方法 :制作日本大耳兔、Wistar大鼠及小鼠的 MODS模型 ,观察联合应用山莨菪碱及地塞米松后动物肿瘤坏死因子 ( TNF)、脂质过氧化物 ( L PO)、组织病理学、胃动素、胃泌素、肾脏内皮素 1( ET 1) m RNA、胃粘膜内 p H值 ( p Hi)的变化。结果 :山莨菪碱及地塞米松联用可使 MODS动物血浆 TNF水平由 ( 147.2 3± 43.93)μmol/ L下降到 ( 6 4.91±2 0 .18)μm ol/ L ,血浆 L PO水平由 ( 8.0 5± 2 .18)μm ol/ L下降到 ( 2 .0 2± 0 .6 4)μmol/ L ,胃粘膜内 p H值从 6 .90± 0 .10增加到 7.30± 0 .10 ,血浆胃泌素水平由 ( 40 6 .3± 5 0 .6 ) pg/ L 降低到 ( 2 10 .4± 43.8) pg/ L,胃动素水平由( 2 2 5 .6± 41.6 ) pg/ L 下降到 ( 143.6± 35 .4) pg/ L,肾脏 ET 1m RNA表达水平由 0 .5 9± 0 .15降低至 0 .39±0 .0 8;注射内毒素后 72小时小鼠死亡率由 6 0 %降至 30 %。结论 :山莨菪碱联用地塞米松可以改善 MODS动物多个器官的功能 ,可以作为治疗 MODS的一种有效方法。
Objective:To study the effect of anisodamine combined with dexamethasone on multiple organ dysfunction syndrome (MODS) and its potential mechanism.Methods:MODS models were reproduced in rabbits and rats,and animals were treated with anisodamine combined with dexamethasone.Changes in endothelin1 (ET1) mRNA expression,plasma gastrin,motilin,tumor necrosis factor (TNF),lipid peroxidation(LPO) levels,and pH values in gastric mucosa were observed in the present study.Results:Following treatment with anisodamine combined with dexamethasone,plasma TNF levels decreased from (147 23±43 93)μmol/L to (64 91±20 18)μmol/L,LPO levels from(8 05±2 18)μmol/L to (2 02±0 64)μmol/L,but pH values in gastric mucosa increased from 6 90±0 10 to 7 30±0 10.Meanwhile,plasma gastrin levels decreased from (406 3±50 6)pg/L to (210 4±43 8)pg/L,motilin levels from (225 6±41 6)pg/L to (143 6±35 4)pg/L,and ET1 mRNA expression in kidney form 0 59±0 15 to 0 39±0 08.The mortality of rats in which endotoxin were injected after 72 hours decreased from 60% to 30%.Conclusions:The combined use of anisodamine with dexamethasone can inhibit inflammatory mediator formation and attenuate multiple organ damage,and it may be a useful method for treatment of MODS.
出处
《中国危重病急救医学》
CSCD
2000年第6期341-343,共3页
Chinese Critical Care Medicine
基金
解放军总装备部卫生局重点科研基金!资助项目(95 2 10 86 )
